○種別 (必須): | □ | 学術論文 (審査論文)
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○言語 (必須): | □ | 英語
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○組織 (推奨): | 1. | 徳島大学.工学部.生物工学科.生物機能工学講座 (〜2023年3月31日)
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○著者 (必須): | 1. | (英) Teramoto Naozumi (日) (読)
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| 2. | (英) Imanishi Yukio (日) (読)
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| 3. | 伊藤 嘉浩
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○題名 (必須): | □ | (英) In vitro selection of a ligase ribozyme carrying alkylamino groups in the side chains (日)
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○副題 (任意): |
○要約 (任意): | □ | (英) A novel ligase ribozyme was in vitro selected from a random-sequence RNA library including N(6)-aminohexyl-modified adenine residues in place of natural adenine residues. This ribozyme mediated the formation of a phosphodiester bond with a DNA oligonucleotide through condensation with a 5'-triphosphate moiety on the ribozyme. Among the clones isolated from this selection, one was shown to accelerate ligation about 250-fold compared to the original random-sequence RNA library. Almost no rate acceleration was observed when the N(6)-aminohexyl-groups on adenine residues were omitted. Furthermore, ligation was dependent on the presence of a template DNA oligonucleotide that bridged the two strands. (日)
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○キーワード (推奨): | 1. | (英) Base Sequence (日) (読)
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| 2. | (英) Catalysis (日) (読)
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| 3. | (英) Cloning, Molecular (日) (読)
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| 4. | (英) Ligases (日) (読)
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| 5. | (英) Molecular Sequence Data (日) (読)
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| 6. | (英) Nucleic Acid Conformation (日) (読)
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| 7. | (英) Oligodeoxyribonucleotides (日) (読)
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| 8. | (英) RNA, Catalytic (日) (読)
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○発行所 (推奨): |
○誌名 (必須): | □ | Bioconjugate Chemistry ([アメリカ化学会])
(pISSN: 1043-1802, eISSN: 1520-4812)
○ISSN (任意): | □ | 1043-1802
ISSN: 1043-1802
(pISSN: 1043-1802, eISSN: 1520-4812) Title: Bioconjugate chemistryTitle(ISO): Bioconjug ChemPublisher: American Chemical Society (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login])
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○巻 (必須): | □ | 11
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○号 (必須): | □ | 6
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○頁 (必須): | □ | 744 748
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○年月日 (必須): | □ | 西暦 2000年 1月 1日 (平成 12年 1月 1日)
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○URL (任意): |
○DOI (任意): | □ | 10.1021/bc990146r (→Scopusで検索)
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○PMID (任意): | □ | 11087320 (→Scopusで検索)
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○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 2. | (英) MedlineDate: 2000 Nov-Dec (日)
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