『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=124345)

EID=124345EID:124345, Map:0, LastModified:2016年9月9日(金) 12:01:43, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[[学科長]/[徳島大学.歯学部.歯学科]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨):
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学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.内田 大亮
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2. (英) Kawamata Hitoshi (日) (読)
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3.表原 文江
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4. (英) Miwa Yoshihiro (日) (読)
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5. (英) Hino Satoshi (日) (読)
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6. (英) Begum Nasima Mila (日) (読)
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7.吉田 秀夫
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8.佐藤 光信
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題名 (必須): (英) Over-expression of TSC-22 (TGF-beta stimulated clone-22) markedly enhances 5-fluorouracil-induced apoptosis in a human salivary gland cancer cell line  (日)    [継承]
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要約 (任意): (英) We have recently isolated TSC-22 (transforming growth factor-beta-stimulated clone-22) cDNA as an anticancer, drug-inducible (with vesnarinone) gene in a human salivary gland cancer cell line, TYS. We have also reported that TSC-22 negatively regulates the growth of TYS cells and that down-regulation of TSC-22 in TYS cells plays a major role in salivary gland tumorigenesis (Nakashiro et al, 1998). In this study, we transfected TYS cells with an expression vector encoding the TSC-22-GFP (green fluorescent protein) fusion protein, and we established TSC-22-GFP-expressing TYS cell clones. Next, we examined (a) the subcellular localization of the fusion protein, (b) the sensitivity of the transfectants to several anticancer drugs (5-fluorouracil, cis-diaminedichloroplatinum, peplomycin), and (c) induction of apoptotic cell death in the transfectants by 5-fluorouracil treatment. The TSC-22-GFP fusion protein was clearly localized to the cytoplasm, but not to the nucleus. Over-expression of the TSC-22-GFP fusion protein did not affect cell growth, but significantly increased the sensitivity of the cells to the anticancer drugs (p < 0.01; one-way ANOVA). Furthermore, over-expression of the TSC-22-GFP fusion protein markedly enhanced 5-fluorouracil-induced apoptosis. These findings suggest that over-expression of TSC-22-GFP protein in TYS cells enhances the chemosensitivity of the cells via induction of apoptosis.  (日)    [継承]
キーワード (推奨): 1. (英) Antineoplastic Agents (日) (読) [継承]
2.アポトーシス (apoptosis) [継承]
3. (英) Cell Adhesion (日) (読) [継承]
4.細胞分裂 (cell division) [継承]
5. (英) Cell Survival (日) (読) [継承]
6. (英) Chromatin (日) (読) [継承]
7. (英) Cisplatin (日) (読) [継承]
8. (英) Fluorouracil (日) (読) [継承]
9. (英) Green Fluorescent Proteins (日) (読) [継承]
10. (英) Humans (日) (読) [継承]
11. (英) Luminescent Proteins (日) (読) [継承]
12. (英) Peplomycin (日) (読) [継承]
13. (英) Recombinant Fusion Proteins (日) (読) [継承]
14. (英) Salivary Gland Neoplasms (日) (読) [継承]
15. (英) Transcription, Genetic (日) (読) [継承]
16. (英) Transfection (日) (読) [継承]
17. (英) Transforming Growth Factor beta (日) (読) [継承]
18. (英) Tumor Cells, Cultured (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Laboratory Investigation; a Journal of Technical Methods and Pathology (United States and Canadian Academy of Pathology)
(pISSN: 0023-6837, eISSN: 1530-0307)

ISSN (任意): 0023-6837
ISSN: 0023-6837 (pISSN: 0023-6837, eISSN: 1530-0307)
Title: Laboratory investigation; a journal of technical methods and pathology
Title(ISO): Lab. Invest.
Publisher: Nature Publishing Group
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2000年 6月 初日 (平成 12年 6月 初日) [継承]
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PMID (任意): 10879745    (→Scopusで検索) [継承]
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WOS (任意): 000087755100016 [継承]
Scopus (任意): 2-s2.0-0033946261 [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Daisuke Uchida, Hitoshi Kawamata, Fumie Omotehara, Yoshihiro Miwa, Satoshi Hino, Mila Nasima Begum, Hideo Yoshida and Mitsunobu Sato : Over-expression of TSC-22 (TGF-beta stimulated clone-22) markedly enhances 5-fluorouracil-induced apoptosis in a human salivary gland cancer cell line, Laboratory Investigation; a Journal of Technical Methods and Pathology, Vol.80, No.6, 955-963, 2000.
欧文冊子 ● Daisuke Uchida, Hitoshi Kawamata, Fumie Omotehara, Yoshihiro Miwa, Satoshi Hino, Mila Nasima Begum, Hideo Yoshida and Mitsunobu Sato : Over-expression of TSC-22 (TGF-beta stimulated clone-22) markedly enhances 5-fluorouracil-induced apoptosis in a human salivary gland cancer cell line, Laboratory Investigation; a Journal of Technical Methods and Pathology, Vol.80, No.6, 955-963, 2000.

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