| ○種別 (必須): | □ | 学術論文 (審査論文)
| [継承] |
| ○言語 (必須): | □ | 英語
| [継承] |
| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.工学部.生物工学科.生物反応工学講座 (〜2023年3月31日)
| [継承] |
| 2. | 独立行政法人 産業技術総合研究所
| [継承] |
| ○著者 (必須): | 1. | (英) Shimizu Yasuhiro (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 2. | 櫻庭 春彦
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 3. | 川上 竜巳 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 4. | 郷田 秀一郎 ([長崎大学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 5. | 河原林 裕 (独立行政法人産業技術総合研究所生物機能工学研究部門)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 6. | 大島 敏久 ([九州大学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| ○題名 (必須): | □ | (英) L-Threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3 (日)
| [継承] |
| ○副題 (任意): | □ | (英) gene cloning and enzymatic characterization (日)
| [継承] |
| ○要約 (任意): | □ | (英) A gene encoding the L-threonine dehydrogenase homologue has been identified in a hyperthermophlic archaeon Pyrococcus horikoshii OT3 via genome sequencing. The gene was cloned and expressed in Escherichia coli. The purified enzyme from the recombinant E. coli was extremely thermostable; the activity was not lost after incubation at 100 degrees C for 20 min. The enzyme (molecular mass: 192 kDa) is composed of a tetrameric structure with a type of subunit (41 kDa). The enzyme is specific for NAD and utilizes L-threonine, L-serine and DL-threo-3-phenylserine as the substrate. The enzyme required divalent cations such as Zn(2+), Mn(2+) and Co(2+) for the activity, and contained one zinc ion/subunit. The K(m) values for L-threonine and NAD at 50 degrees C were 0.20 mM and 0.024 mM, respectively. Kinetic analyses indicated that the L-threonine oxidation reaction proceeds via a random mechanism with regard to the binding of L-threonine and NAD. The enzyme showed pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of NADH. This is the first description of the characteristics of an L-threonine dehydrogenase from the archaea domain. (日)
| [継承] |
| ○キーワード (推奨): | 1. | (英) Alcohol Oxidoreductases (日) (読)
| [継承] |
| 2. | (英) Amino Acid Sequence (日) (読)
| [継承] |
| 3. | (英) Animals (日) (読)
| [継承] |
| 4. | (英) Catalysis (日) (読)
| [継承] |
| 5. | (英) Cloning, Molecular (日) (読)
| [継承] |
| 6. | (英) Hydrogen (日) (読)
| [継承] |
| 7. | (英) Magnetic Resonance Spectroscopy (日) (読)
| [継承] |
| 8. | (英) Molecular Sequence Data (日) (読)
| [継承] |
| 9. | (英) NADP (日) (読)
| [継承] |
| 10. | (英) Pyrococcus horikoshii (日) (読)
| [継承] |
| 11. | (英) Recombinant Proteins (日) (読)
| [継承] |
| 12. | (英) Sequence Alignment (日) (読)
| [継承] |
| 13. | (英) Sequence Homology, Amino Acid (日) (読)
| [継承] |
| 14. | (英) Serine (日) (読)
| [継承] |
| 15. | (英) Stereoisomerism (日) (読)
| [継承] |
| 16. | (英) Substrate Specificity (日) (読)
| [継承] |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Extremophiles (International Society for Extremophiles)
(pISSN: 1431-0651, eISSN: 1433-4909)
| ○ISSN (任意): | □ | 1431-0651
ISSN: 1431-0651
(pISSN: 1431-0651, eISSN: 1433-4909) Title: Extremophiles : life under extreme conditionsTitle(ISO): ExtremophilesSupplier: Springer Online Journal ArchivePublisher: Springer (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
| [継承] |
| ○巻 (必須): | □ | 9
| [継承] |
| ○号 (必須): | □ | 4
| [継承] |
| ○頁 (必須): | □ | 317 324
| [継承] |
| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2005年 8月 初日 (平成 17年 8月 初日)
| [継承] |
| ○URL (任意): | □ | http://ci.nii.ac.jp/naid/80017783245/
| [継承] |
| ○DOI (任意): | □ | 10.1007/s00792-005-0447-2 (→Scopusで検索)
| [継承] |
| ○PMID (任意): | □ | 15902509 (→Scopusで検索)
| [継承] |
| ○CRID (任意): | □ | 1571417126449425536
| [継承] |
| ○NAID : | □ | 80017783245
| [継承] |
| ○WOS (任意): | □ | 000231020800007
| [継承] |
| ○Scopus (任意): |
| ○researchmap (任意): |
| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.Affiliation: Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, 2-1 Minamijosanjimacho, Tokushima, Japan. (日)
| [継承] |
| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
| [継承] |
| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
| [継承] |