『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
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組織 (推奨): 1.株式会社 中山書店 [継承]
2.徳島大学.歯学部.歯学科.基礎口腔科学講座.分子薬理学 (歯科薬理学講座) (〜2020年6月30日) [継承]
著者 (必須): 1.石川 康子
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[継承]
2. (英) Yuan Zhenfang (日) (読)
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[継承]
3. (英) Inoue Noriko (日) (読)
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[継承]
4. (英) Skowronski T. Mariusz (日) (読)
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[継承]
5.中江 良子
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6.庄野 正行 ([徳島大学.大学院医歯薬学研究部.医学域.総合研究支援センター])
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7. (英) Cho Gota (日) (読)
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8. (英) Yasui Masato (日) (読)
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9. (英) Agre Peter (日) (読)
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[継承]
10. (英) Nielsen Soren (日) (読)
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[継承]
題名 (必須): (英) Identification of aquaporin-5 in lipid rafts and its translocation to apical membranes by the activation of M3-muscarinic acetylcholine receptors in the interlobular ducts of rat parotid glands  (日)    [継承]
副題 (任意):
要約 (任意): (英) Aquaporin-5 (AQP5), an apical plasma membrane (APM) water channel in salivary glands, lacrimal glands, and airway epithelium, has an important role in fluid secretion. M(3) muscarinic acetylcholine receptor (mAChR)-induced changes in AQP5 localization in rat parotid glands were investigated with immunofluorescence or immunoelectron microscopy, detergent solubility, and gradient density floatation assays. Confocal microscopy revealed AQP5 localization in intracellular vesicles of interlobular duct cells in rat parotid glands and AQP5 trafficking to the APM 10 min after injection of the mAChR agonist cevimeline. Conversely, 60 min after injection, there was a diffuse pattern of AQP5 staining in the cell cytoplasm. The calcium ionophore A-23187 mimicked the effects of cevimeline. Immunoelectron microscopic studies confirmed that cevimeline induced AQP5 trafficking from intracellular structures to APMs in the interlobular duct cells of rat parotid glands. Lipid raft markers flotillin-2 and GM1 colocalized with AQP5 and moved with AQP5 in response to cevimeline. Under control conditions, the majority of AQP5 localized in the Triton X-100-insoluble fraction and floated to the light-density fraction on discontinuous density gradients. After 10-min incubation of parotid tissue slices with cevimeline or A-23187, AQP5 levels decreased in the Triton X-100-insoluble fraction and increased in the Triton X-100-soluble fraction. Thus AQP5 localizes in the intracellular lipid rafts, and M(3) mAChR activation induces AQP5 trafficking to the APM with lipid rafts via intracellular Ca(2+) signaling and induces AQP5 dissociation from lipid rafts to nonrafts on the APM in the interlobular duct cells of rat parotid glands.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Aquaporin 5 (日) (読) [継承]
3. (英) Aquaporins (日) (読) [継承]
4.カルシウム (calcium) [継承]
5. (英) Enzyme Activation (日) (読) [継承]
6. (英) Male (日) (読) [継承]
7. (英) Membrane Microdomains (日) (読) [継承]
8. (英) Membrane Proteins (日) (読) [継承]
9. (英) Parotid Gland (日) (読) [継承]
10. (英) Protein Transport (日) (読) [継承]
11. (英) Quinuclidines (日) (読) [継承]
12. (英) Rats (日) (読) [継承]
13. (英) Rats, Wistar (日) (読) [継承]
14. (英) Receptor Protein-Tyrosine Kinases (日) (読) [継承]
15. (英) Thiophenes (日) (読) [継承]
発行所 (推奨):
誌名 (必須): American Journal of Physiology, Cell Physiology ([アメリカ生理学会])
(pISSN: 0363-6143, eISSN: 1522-1563)

ISSN (任意): 0363-6143
ISSN: 0363-6143 (pISSN: 0363-6143, eISSN: 1522-1563)
Title: American journal of physiology. Cell physiology
Title(ISO): Am J Physiol Cell Physiol
Publisher: American Physiological Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 289 [継承]
(必須): 5 [継承]
(必須): C1303 C1311 [継承]
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年月日 (必須): 西暦 2005年 11月 初日 (平成 17年 11月 初日) [継承]
URL (任意):
DOI (任意): 10.1152/ajpcell.00211.2005    (→Scopusで検索) [継承]
PMID (任意): 16107506    (→Scopusで検索) [継承]
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WOS (任意): 000232385300027 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Medical Pharmacology, Institute of Health Biosciences, University of Tokushima Graduate School, 3-18-15, Kuramoto-cho, Tokushima 770-8504, Japan. isikawa@dent.tokushima-u.ac.jp  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Yasuko Ishikawa, Zhenfang Yuan, Noriko Inoue, Mariusz T. Skowronski, Yoshiko Nakae, Masayuki Shono, Gota Cho, Masato Yasui, Peter Agre and Soren Nielsen : Identification of aquaporin-5 in lipid rafts and its translocation to apical membranes by the activation of M3-muscarinic acetylcholine receptors in the interlobular ducts of rat parotid glands, American Journal of Physiology, Cell Physiology, Vol.289, No.5, C1303-C1311, 2005.
欧文冊子 ● Yasuko Ishikawa, Zhenfang Yuan, Noriko Inoue, Mariusz T. Skowronski, Yoshiko Nakae, Masayuki Shono, Gota Cho, Masato Yasui, Peter Agre and Soren Nielsen : Identification of aquaporin-5 in lipid rafts and its translocation to apical membranes by the activation of M3-muscarinic acetylcholine receptors in the interlobular ducts of rat parotid glands, American Journal of Physiology, Cell Physiology, Vol.289, No.5, C1303-C1311, 2005.

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