『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=121859)

EID=121859EID:121859, Map:0, LastModified:2017年12月4日(月) 13:55:32, Operator:[[ADMIN]], Avail:TRUE, Censor:0, Owner:[[学科長]/[徳島大学.工学部.化学応用工学科]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (紀要その他) [継承]
言語 (必須): 日本語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.工学部.生物工学科.生物反応工学講座 [継承]
2.徳島大学.工学部.化学応用工学科 [継承]
著者 (必須): 1.郷田 秀一郎 ([長崎大学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.薮谷 智規
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3.櫻庭 春彦
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4.本仲 純子
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5.大島 敏久 ([九州大学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Development of Biosensors Using Hyperthermophilic Enzymes as an Element:Development of Biosensors Using Hyperthermophilic Enzymes as an Element:Functional Analysis of Polyamine Metabolizing Enzymes and Development for EnzymeElectrode Sensor Using D-Proline Dehydrogenases  (日) 超好熱菌酵素を素子とするバイオセンサーの開発:ポリアミン関連酵素の機能解析とD-プロリン脱水素酵素機能電極センサーの開発   [継承]
副題 (任意):
要約 (任意): (英) An amperometric enzyme sensor give us higher sensitivity and specificity for the substratedetermination. In spite of advantages of enzyme sensor, many enzymes so far found have been toolabile to use as biosensor elements in artificial circumstances for a longer period.Hyperthermophiles, which can grow above 90℃, have been known to produce much more stableenzymes under various artificial conditions. In this work, we carried out screening, biochemicalcharacterization and improvement of production for hyperthermostable enzymes which are more usefulas the elements in the biosensors. We focused on the polyamines as one of the substrates ofbiosensors. Polyamines have been known to play many important roles in cell proliferation,differentiation and transformation. The concentration of the polyamines together with their acetylconjugates remarkably increases in the biological fluids and the affected tissues of cancer patients.Therefore, their polyamines are listed as tumor markers. Gas and ion chromatographies have beenso far used for polyamine determination, but have some problems from the aspects of high sensitivityand easy operation. Thus, we here developed biosensors using hyperthermostable enzymes forpolyamine determination. Such enzyme sensors are more useful for the simple and rapiddetermination of polyamines and application for clinical analysis and food analysis In addition, wetried the construction of biosensor using the hyperthermophilic enzyme, D-Proline dehydrogenase.As the results, we found the thermostable agmatinase and spermidine dehydrogenase inhyperthermophiles, Pyrococcus horikoshii and Sulfolobus tokodaii, respectively. We succeeded theconstruction of novel amperometric sensor for D-proline determination using D-Proline dehydrogenasederived from Pyrobaculum islandicum.  (日)    [継承]
キーワード (推奨): 1. (英) enzyme sensor, hyperthermophile, dehydrogenase, polyamine (日) (読) [継承]
発行所 (推奨): 徳島大学 [継承]
誌名 (必須): 徳島大学工学部研究報告 ([徳島大学.工学部])
(pISSN: 0371-5949)

ISSN (任意): 0371-5949
ISSN: 0371-5949 (pISSN: 0371-5949)
Title: 徳島大学工学部研究報告
Supplier: 徳島大学
 (Webcat Plus (No Scopus information.)
[継承]
[継承]
(必須): 50 [継承]
(必須): [継承]
(必須): [継承]
都市 (任意):
年月日 (必須): 西暦 2005年 3月 初日 (平成 17年 3月 初日) [継承]
URL (任意): http://ci.nii.ac.jp/naid/110001835850/ [継承]
DOI (任意):
PMID (任意):
NAID (任意): 110001835850 [継承]
WOS (任意):
Scopus (任意):
機関リポジトリ : 45160 [継承]
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意):

標準的な表示

和文冊子 ● 郷田 秀一郎, 薮谷 智規, 櫻庭 春彦, 本仲 純子, 大島 敏久 : 超好熱菌酵素を素子とするバイオセンサーの開発:ポリアミン関連酵素の機能解析とD-プロリン脱水素酵素機能電極センサーの開発, 徳島大学工学部研究報告, Vol.50, 2005年.
欧文冊子 ● Shuichiro Goda, Tomoki Yabutani, Haruhiko Sakuraba, Junko Motonaka and Toshihisa Ohshima : Development of Biosensors Using Hyperthermophilic Enzymes as an Element:Development of Biosensors Using Hyperthermophilic Enzymes as an Element:Functional Analysis of Polyamine Metabolizing Enzymes and Development for EnzymeElectrode Sensor Using D-Proline Dehydrogenases, Bulletin of Faculty of Engineering, The University of Tokushima, Vol.50, 2005.

関連情報

Number of session users = 0, LA = 0.62, Max(EID) = 383954, Max(EOID) = 1027007.