『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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組織 (推奨): 1.徳島大学.分子酵素学研究センター (〜2007年3月31日/->組織[徳島大学.疾患酵素学研究センター]) [継承]
著者 (必須): 1. (英) Hayashi Hideki (日) (読)
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2. (英) Miyake Noriaki (日) (読)
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3. (英) Kanai Fumihiko (日) (読)
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4. (英) Shibasaki Futoshi (日) (読)
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5. (英) Takenawa Tadaomi (日) (読)
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6.蛯名 洋介
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題名 (必須): (英) Phosphorylation in vitro of the 85 kDa subunit of phosphatidylinositol 3-kinase and its possible activation by insulin receptor tyrosine kinase.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Insulin causes a dramatic and rapid increase in phosphatidylinositol 3-kinase activity in the anti-phosphotyrosine immunoprecipitates of cells overexpressing the human insulin receptor. This enzyme may therefore be a mediator of insulin signal transduction [Endemann, Yonezawa & Roth (1990) J. Biol. Chem. 265, 396-400; Ruderman, Kapeller, White & Cantley (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 1411-1415]. At least two questions remain to be elucidated. Firstly, does the insulin receptor tyrosine kinase phosphorylate phosphatidylinositol 3-kinase directly, or does it phosphorylate a protein associated with the 3-kinase? Second, if the enzyme is a direct substrate for the insulin receptor tyrosine kinase, does tyrosine phosphorylation of phosphatidylinositol 3-kinase by the kinase alter the specific enzyme activity, or does the amount of the tyrosine-phosphorylated form of the phosphatidylinositol 3-kinase increase, with no change in the specific activity? We report here evidence that the 85 kDa subunit of highly purified phosphatidylinositol 3-kinase is phosphorylated on the tyrosine residue by the activated normal insulin receptor in vitro, but not by a mutant insulin receptor which lacks tyrosine kinase activity. We found that an increase in enzyme activity was detected in response to insulin not only in the anti-phosphotyrosine immunoprecipitates of the cytosol, but also in the cytosolic fraction before immunoprecipitation. In addition, we partially separated the tyrosine-phosphorylated form from the unphosphorylated form of the enzyme, by using a f.p.l.c. Mono Q column. The insulin-stimulated phosphatidylinositol 3-kinase activity was mainly detected in the fraction containing almost all of the tyrosine-phosphorylated form. This result suggests that tyrosine phosphorylation of phosphatidylinositol 3-kinase by the insulin receptor kinase may increase the specific activity of the former enzyme in vivo.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) CHO Cells (日) (読) [継承]
3. (英) Cricetinae (日) (読) [継承]
4. (英) Cytosol (日) (読) [継承]
5. (英) Enzyme Activation (日) (読) [継承]
6. (英) Humans (日) (読) [継承]
7.インスリン (insulin) [継承]
8. (英) Macromolecular Substances (日) (読) [継承]
9. (英) Phosphatidylinositol 3-Kinases (日) (読) [継承]
10.リン酸化 (phosphorylation) [継承]
11. (英) Phosphotransferases (日) (読) [継承]
12. (英) Phosphotyrosine (日) (読) [継承]
13. (英) Protein-Tyrosine Kinases (日) (読) [継承]
14. (英) Receptor, Insulin (日) (読) [継承]
15. (英) Tyrosine (日) (読) [継承]
発行所 (推奨): The Biochemical Society [継承]
誌名 (必須): The Biochemical Journal ([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)

ISSN (任意): 0264-6021
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 280 [継承]
(必須): Pt 3 [継承]
(必須): 769 775 [継承]
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年月日 (必須): 西暦 1991年 12月 15日 (平成 3年 12月 15日) [継承]
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PMID (任意): 1722393    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Enzyme Genetics, University of Tokushima, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: In Vitro  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) OtherID: PMC1130520  (日)    [継承]

標準的な表示

和文冊子 ● Hideki Hayashi, Noriaki Miyake, Fumihiko Kanai, Futoshi Shibasaki, Tadaomi Takenawa and Yousuke Ebina : Phosphorylation in vitro of the 85 kDa subunit of phosphatidylinositol 3-kinase and its possible activation by insulin receptor tyrosine kinase., The Biochemical Journal, Vol.280, No.Pt 3, 769-775, 1991.
欧文冊子 ● Hideki Hayashi, Noriaki Miyake, Fumihiko Kanai, Futoshi Shibasaki, Tadaomi Takenawa and Yousuke Ebina : Phosphorylation in vitro of the 85 kDa subunit of phosphatidylinositol 3-kinase and its possible activation by insulin receptor tyrosine kinase., The Biochemical Journal, Vol.280, No.Pt 3, 769-775, 1991.

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