Takeshi Omasa, Masayoshi OnitsukaandWook-Dong Kim : Cell engineering and cultivation of Chinese hamster ovary (CHO) cells, Current Pharmaceutical Biotechnology, Vol.11, No.3, 232-240, April 2010..[著者]
Masayoshi Onitsuka, Wook-Dong Kim, Hiroyuki Ozaki, Akira Kawaguchi, Kohsuke Honda, Hisao OhtakeandTakeshi Omasa : Improvement of glycosylation pattern of humanized IgG-like bispecificantibody produced by recombinant CHO cells, 23th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT '10), 97, Sappporo, Sep. 2010..[著者]
Masayoshi Onitsuka, Wook-Dong Kim, Hiroyuki Ozaki, Akira Kawaguchi, Kohsuke Honda, Hisao OhtakeandTakeshi Omasa : Improvement of glycosylation pattern of humanized IgG-like bispecificantibody produced by recombinant CHO cells, 23th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT '10), 170, Sappporo, Sep. 2010..[著者]
Takeshi Omasa, Masayoshi OnitsukaandYihua Cao : Next Generation Mammalian Host Cell for Biopharmaceutical Production, Asian Congress on Biotechnology, 97, Shanghai, May 2011..[著者]
Akihiro Shirai, kuboyama Yasunori, Masayoshi Onitsuka, Katsu Satoshi, Takami Takayuki, Hideaki MasedaandTakeshi Omasa : The effects of CHOP over-expression/down-regulation on recombinant Antithrombin III production in Chinese Hamster Ovary Cells, The 17th Young Asian Biochemical Engineers' Community (YABEC), No.F-8, 120, Incheon, Oct. 2011..[著者]
Masayoshi Onitsuka, Tatsuzawa Miki, Akihiro Shirai, Hideaki MasedaandTakeshi Omasa : Effects of trehalose on antibody production: Supression of protein aggregation and enhancement of productivity, The 17th Young Asian Biochemical Engineers' Community (YABEC), No.F-9, 121, Incheon, Oct. 2011..[著者]
Masayoshi Onitsuka, Wook-Dong Kim, H. Ozaki, Akira Kawaguchi, Kohsuke Honda, H. Kajiura, K. Fujiyama, Ryutaro Asano, Izumi Kumagai, Hisao OhtakeandTakeshi Omasa : Enhancement of sialylation on humanized lgG-like bispecific antibody by overexpression of 2,6-sialyltransferase derived from Chainese hamster ovary cells, Applied Microbiology and Biotechnology, Vol.94, No.1, 69-80, 2012..[著者]
Akihiro Shirai, Koichiro Sato, Masayoshi Onitsuka, Hideaki MasedaandTakeshi Omasa : The effect of WFS1 over-expression on recombinant protein production in Chinese Hamster Ovary Cells, The 18th Young Asian Biochemical Engineers' Community (YABEC), 113, Tokushima, Oct. 2012..[著者]
Masayoshi Onitsuka, Akira Kawaguchi, Miki Tatsuzawa, Kohsuke Honda, Hisao OhtakeandTakeshi Omasa : Deglycosylation induces antibody aggregation in culture process of Chinese hamster ovary cell, The 18th Young Asian Biochemical Engineers' Community (YABEC), 114, Tokushima, Oct. 2012..[著者]
Mai Takahashi, Syuichi Kimura, Haphparast Mohammad Ali Seyed, Yihua Cao, Takayuki Itoi, Akihiro Shirai, Masayoshi OnitsukaandTakeshi Omasa : Clonal variability and chromosomal heterogeneity in Chinese hamster ovary cell lines during long-term cultivation, The 18th Young Asian Biochemical Engineers' Community (YABEC), 117, Tokushima, Oct. 2012..[著者]
Michiko Hoashi, Mai Takahashi, Masayoshi Onitsuka, Akihiro Shirai, Hideaki MasedaandTakeshi Omasa : Construction of new mammalian expression vector on the basis of gene-amplified structure in CHO genome, The 18th Young Asian Biochemical Engineers' Community (YABEC), 118, Tokushima, Oct. 2012..[著者]
Kyongho Lee, Masayoshi Onitsuka, Kohsuke Honda, Hisao OhtakeandTakeshi Omasa : Construction of High-Producing CHO Cell Lines by Controlling Cell Cycle Checkpoint, The 18th Young Asian Biochemical Engineers' Community (YABEC), 119, Tokushima, Oct. 2012..[著者]
Masayoshi Onitsuka, Miki Tatsuzawa, Kazuo Okuyama, Ichiro Koguma, Akihiro ShiraiandTakeshi Omasa : Purification and aggregation analysis of humanized IgG-like bispecific diabody-Fc with thermo responsive Protein A (TRPA) column, 25th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT 2012), 111, Nagoya, Nov. 2012..[著者]
Masayoshi Onitsuka, Akihiro ShiraiandTakeshi Omasa : Rapid evalution of glycosylation CHO antibody production, 25th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT 2012), 110, Nagoya, Nov. 2012..[著者]
Miki Tatsuzawa, Masayoshi Onitsuka, Akihiro Shirai, Hideaki MasedaandTakeshi Omasa : Trehalose suppress the antibody aggregation in CHO cell culture, 25th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT 2012), 106, Nagoya, Nov. 2012..[著者]
Tomomi Tsutsui, Akitoshi Nishizawa, Akihiro Shirai, Masayoshi Onitsuka, Hideaki MasedaandTakeshi Omasa : Expression profiling in UPR-regulated Chinese hamster ovary cell line, 25th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT 2012), 107, Nagoya, Nov. 2012..[著者]
Mai Takahashi, Syuichi Kimura, Haghparast Mohammad Ali Seyed, Yihua Cao, Takayuki Itoi, Akihiro Shirai, Masayoshi OnitsukaandTakeshi Omasa : Relationship between chromosomal instability an cell characterization in Chinese hamster ovary cell line, 25th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT 2012), 108, Nagoya, Nov. 2012..[著者]
Kyongho Lee, Masayoshi Onitsuka, Kohsuke Honda, Hisao OhtakeandTakeshi Omasa : Accelerated gene amplification by cell cycle checkpoint engineering and its industrial applications, 25th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT 2012), 116, Nagoya, Nov. 2012..[著者]
Lee Ho Kyoung, Masayoshi Onitsuka, Kohsuke Honda, Hisao OhtakeandTakeshi Omasa : Rapid construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering, Applied Microbiology and Biotechnology, Vol.97, No.13, 5731-5741, 2013..[著者]
Masayoshi Onitsuka, Tatsuzawa Miki, Noda Masahiro, Koguma Ichiro, Akihiro ShiraiandTakeshi Omasa : Dynamical analysis of antibody aggregation in the CHO cell culture with thermo responsive protein A (TRPA) column, ESACT Meeting 2013 in Lille, No.A122, 97, (都市), June 2013..[著者]
Noda Masahiro, Tatsuzawa Miki, Masayoshi Onitsuka, Akihiro ShiraiandTakeshi Omasa : Chemical shaperon suppresses the antibody aggregation in CHO cell culture, ESACT Meeting 2013 in Lille, No.A121, 97, Lille,France, June 2013..[著者]
Tomomi Tsutsui, Ho Kyong Lee, Rina Matsuyama, Masayoshi OnitsukaandTakeshi Omasa : Cell cycle checkpoint engineering: novel construction method of gene-amplified CHO cell line for therapeutic antibody production, The 12th Tunisian-Japanese Symposium on Society, Science and Technology (TJASSST2013), 14, Tsunisia, Nov. 2013..[著者]
Masayoshi Onitsuka, Akira Kawaguchi, Ryutaro Asano, Izumi Kumagai, Kohsuke Honda, Hisao OhtakeandTakeshi Omasa : Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture, Journal of Bioscience and Bioengineering, Vol.117, No.5, 639-644, 2014..[著者]
Akihiro Shirai, Matsumura Kyohei, Masayoshi Onitsuka, Hideaki MasedaandTakeshi Omasa : Application of photochromism to the molecular design of antimicrobial agents: synthesis of phenolic derivatives and their bactericidal activity based on a photo-reaction with ultraviolet-A light, III International Conference on Antimicrobial Research, No.T64, 289, Madrid, Oct. 2014..[著者]
Noriko Yamano, Mai Takahashi, Toshitaka Kumamoto, Tomoharu Murakami, Jana Frank, Masayoshi OnitsukaandTakeshi Omasa : The effect of chromosome number to antibody production in Chinese Hamster Ovary Cells, The 20th Symposium of Young Asian Biochemical Engineers' Community (YABEC) 2014, (巻), (号), (頁), (都市), Nov. 2014..[著者]
Jana Frank, Toshitaka Kumamoto, Masayoshi Onitsuka, Noriko YamanoandTakeshi Omasa : Chromosome rearrangements and instability in Chinese hamster ovary cell line, The 20th Symposium of Young Asian Biochemical Engineers' Community (YABEC) 2014, (巻), (号), (頁), (都市), Nov. 2014..[著者]
Matsuyama Rima, Tsutsui Tomomi, Lee Ho Kyoung, Masayoshi OnitsukaandTakeshi Omasa : Accelerated gene amplification by checkpoint bypass with cell division cycle 25 homolog B (CBC25B), 7th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT 2014), P.74, (都市), Nov. 2014..[著者]
Kinoshita Yukie, Masayoshi OnitsukaandTakeshi Omasa : Enhanced antibody production by transcription factor NFKBIZ in CHO cells, 27th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT '14), P.75, Kitakyushu, Japan, Nov. 2014..[著者]
Jana Frank, Kumamoto Toshitaka, Noriko Yamano, Masayoshi OnitsukaandTakeshi Omasa : Function of chromosome fragile sites in engineeiring of Chinese hamster ovary DG44-based cell line, 27th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT '14), P.88, Kitakyushu, Japan, Nov. 2014..[著者]
Masayoshi Onitsuka, Ide TeruhikoandTakeshi Omasa : Separation of antibody aggregation with FcRI column, 27th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT '14), P.89, Kitakyushu, Japan, Nov. 2014..[著者]
Masayoshi OnitsukaandTakeshi Omasa : Antibody Aggregation in CHO Cell Culture: Mechanism and Suppression, Symposium 3 "Advanced Technologies for Next Generation Cell and Cell Culture Engineering'' in 27th Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT 2014), (巻), (号), (頁), (都市), Nov. 2014..[著者]
Masayoshi OnitsukaandTakeshi Omasa : Rapid evaluation of N-glycosylation status of antibodies with chemiluminescent lectin-binding assay., Journal of Bioscience and Bioengineering, Vol.120, No.1, 107-110, 2014..[著者]
Akihiro Shirai, Masayoshi Onitsuka, Hideaki MasedaandTakeshi Omasa : Effect of polyphenols on reactive oxygen species production and cell growth of human dermal fibroblasts after irradiation with ultraviolet-A light, Biocontrol Science, Vol.20, No.1, 27-33, 2015..[著者]
Toshitaka Kumamoto, Mai Takahashi, Masayoshi OnitsukaandTakeshi Omasa : Analysis of chromosome number and its application to antibody production in Chinese hamster ovary cells., Culture Engineering XIV, Quebec City, Canada, P-18, (都市), May 2015..[著者]
Matsuyama Rima, Tsutsui Tomomi, Lee Ho Kyoung, Masayoshi OnitsukaandTakeshi Omasa : Generation of high-producing cell lines by cell cycle checkpoint engineering in CHO cells., Cell Culture Engineering XIV, Quebec City, Canada, P-44, (都市), May 2015..[著者]
Noriko Yamano, Toshitaka Kumamoto, Mai Takahashi, Jana Frank, Masayoshi OnitsukaandTakeshi Omasa : Stability difference of each chromosome in Chinese Hamster Ovary cell line, The 24th Meeting of the European Society for Animal Cell Technology (ESACT) 2015, (巻), (号), (頁), (都市), May 2015..[著者]
YuanShan Lai, Noriko Yamano, Masayoshi OnitsukaandTakeshi Omasa : Increased antibody productivity in Chinese Hamster Ovary cells through induction of chromosomal instability by cell fusion, The 24th Meeting of the European Society for Animal Cell Technology (ESACT) 2015, (巻), (号), (頁), (都市), May 2015..[著者]
Marina Aga, Noriko Yamano, Toshitaka Kumamoto, Jana Frank, Masayoshi OnitsukaandTakeshi Omasa : Construction of a gene knockout CHO cell line using a simple gene targeting method, The 24th Meeting of the European Society for Animal Cell Technology (ESACT) 2015, (巻), (号), (頁), (都市), May 2015..[著者]
Rima Matsuyama, Tomomi Tsutsui, Ho Kyoung Lee, Masayoshi OnitsukaandTakeshi Omasa : Improved gene amplification by cell-cycle engineering combined with the Cre-loxP system in Chinese hamster ovary cells., Journal of Bioscience and Bioengineering, Vol.120, No.6, 701-708, 2015..[著者]
Noriko Yamano, Toshitaka Kumamoto, Mai Takahashi, Jana Frank, Masayoshi OnitsukaandTakeshi Omasa : Stability difference of each chromosome in Chinese Hamster Ovary cell line, BMC Proceedings, Vol.9, No.Supplement 9, P1, 2015..[著者]
Marina Aga, Noriko Yamano, Toshitaka Kumamoto, Jana Frank, Masayoshi OnitsukaandTakeshi Omasa : Construction of a gene knockout CHO cell line using a simple gene targeting method, BMC Proceedings, Vol.9, No.Supplement 9, P2, 2015..[著者]
Noriko Yamano, Toshitaka Kumamoto, Kota Yoshitomi, Jana Frank, Masayoshi OnitsukaandTakeshi Omasa : Varied productivity according to the differences between targeted locations of antibody expression vectors in Chinese Hamster ovary cells, Cell Culture Engineering XV, (巻), (号), (頁), (都市), May 2016..[著者]
Takeshi Omasa, Noriko YamanoandMasayoshi Onitsuka : Mammalian cell factory- CHO cell and its application for biopharmaceutical production, World Congress on In Vitro Biology, (巻), (号), (頁), (都市), June 2016..[著者]
Noriko Yamano, Mai Takahashi, Haghparast Mohammad Ali Seyed, Masayoshi Onitsuka, Toshitaka Kumamoto, Jana FrankandTakeshi Omasa : Increased recombinant protein production owing to expanded opportunities for vector integration in high chromosome number Chinese hamster ovary cells, Journal of Bioscience and Bioengineering, Vol.122, No.2, 226-231, 2016..[著者]
Kohei Kaneyoshi, Keiji Uchiyama, Masayoshi Onitsuka, Noriko Yamano, Yuichi KogaandTakeshi Omasa : Analysis of intracellular recombinant IgG secretion in engineered CHO cells, The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), (巻), (号), (頁), (都市), Nov. 2016..[著者]
Sho Tanaka, Noriko Yamano, Norichika Ogata, Masayoshi Onitsuka, Yuichi KogaandTakeshi Omasa : Genome-wide analysis of gene expression in antibody-producing CHO cells with chromosome aneuploidy, The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), (巻), (号), (頁), (都市), Nov. 2016..[著者]
Kota Yoshitomi, Noriko Yamano, Wataru Tanaka, Masayoshi Onitsuka, Yuichi KogaandTakeshi Omasa : Improved antibody productivities of CHO cells constructed by targeting gene-rich chromosomal regions on the stable chromosome, The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), (巻), (号), (頁), (都市), Nov. 2016..[著者]
Ryonosuke Harata, Masayoshi Onitsuka, Takahiro Kikawada, Shizuyo Koide, Noriko Yamano, Yuichi KogaandTakeshi Omasa : Suppressed antibody aggregation and improved cell growth by exogenous expression of Tret1 in recombinant CHO cells, The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), (巻), (号), (頁), (都市), Nov. 2016..[著者]
Yoshiki Nomura, Masayoshi Onitsuka, Noriko Yamano, Yuichi KogaandTakeshi Omasa : Secretory expression of Immunoglobulin New Antigen Receptor in Chinese Hamster Ovary cells, The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), (巻), (号), (頁), (都市), Nov. 2016..[著者]
Jana Frank, Masayoshi Onitsuka, Noriko YamanoandTakeshi Omasa : Improved protein production of Chinese hamster ovary DG44 cells treated with aphidicolin, The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), (巻), (号), (頁), (都市), Nov. 2016..[著者]
Masayoshi OnitsukaandTakeshi Omasa : Dynamical Analysis of Aggregate Accumulation of IgG1 in recombinant CHO cell Culture, The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe), (巻), (号), (頁), (都市), Nov. 2016..[著者]
K. Kaneyoshi, K. Uchiyama, Masayoshi Onitsuka, Noriko Yamano, Y. KogaandTakeshi Omasa : INTRACELLULAR SECRETION ANALYSIS OF RECOMBINANT THERAPEUTIC ANTIBODIES IN ENGINEERED CHO CELLS AIMING TO ESTABLISH HIGH PRODUCER, The 25th Meeting of the European Society for Animal Cell Technology (ESACT) 2017, Lausanne, Switzerland, May 2017..[著者]
Masayoshi Onitsuka, Y. Fujino, N. KawamuraandTakeshi Omasa : CONSTRUCTION OF A SYSTEM FOR RAPID EVALUATION OF PRODUCTION ENHANCER GENE IN CHO ANTIBODY PRODUCTION, The 25th Meeting of the European Society for Animal Cell Technology (ESACT) 2017, Lausanne, Switzerland, May 2017..[著者]
Noriko Yamano, S. Tanaka, N. Ogata, Masayoshi Onitsuka, Y. KogaandTakeshi Omasa : CHARACTERIZATION OF ANTIBODY-PRODUCING CHO CELLS WITH CHROMOSOME ANEUPLOIDY, The 25th Meeting of the European Society for Animal Cell Technology (ESACT) 2017, Lausanne, Switzerland, May 2017..[著者]
W. Tanaka, K. Yoshitomi, Noriko Yamano, Masayoshi Onitsuka, Y. KogaandTakeshi Omasa : ENHANCEMENT OF ANTIBODY PRODUCTIVITY IN RECOMBINANT CHO CELLS CONSTRUCTED BY TARGETING THE IGG1 GENE TO THE STABLE CHROMOSOME, The 25th Meeting of the European Society for Animal Cell Technology (ESACT) 2017, Lausanne, Switzerland, May 2017..[著者]
K Kaneyoshi, Keiji Uchiyama, Masayoshi Onitsuka, Noriko Yamano, Y KogaandTakeshi Omasa : Intracellular secretion analysis of recombinant therapeutic antibodies in engineered CHO cells aiming to establish high produce., The 25th Meeting of the European Society for Animal Cell Technology (ESACT2017), (都市), May 2017..[著者]
Tang Qing, Masayoshi Onitsuka, Atsushi Tabata, Toshifumi TomoyasuandHideaki Nagamune : Characterizatio of anti-HER2 scFv and Fab as a targeting module for novel drug-delivery system against HER2-positive cancer, 24th IUBMB congress & 15th FAOBMB congress, Seoul, Korea, (都市), June 2018..[著者]
Qing Tang, Masayoshi Onitsuka, Atsushi Tabata, Toshifumi TomoyasuandHideaki Nagamune : Construction of Anti-HER2 Recombinants as Targeting Modules for a Drug-delivery System Against HER2-positive Cells, Anticancer Research, Vol.38, No.7, 4319-4325, 2018..[著者]
Masayoshi Onitsuka, Atsuko ShimazuandHiroe Amou : Extracellular glycosylation for therapeutic antibody production with improved glycoforms, The 31st Annual and International Meeting of the Japanese Association for Animal Cell Technology, (都市), Nov. 2018..[著者]
鬼塚 正義, 本田 真也 : Secretion of an aggregated antibody from recombinant CHO cells revealed by artificial protein sensor AF.2A1, 第19回日本蛋白質科学会年会, 2019年6月..[著者]
Noriko Yamano-Adachi, Norichika Ogata, Sho Tanaka, Masayoshi OnitsukaandTakeshi Omasa : Characterization of Chinese hamster ovary cells with disparate chromosome numbers: Reduction of the amount of mRNA relative to total protein, Journal of Bioscience and Bioengineering, Vol.129, No.1, 121-128, 2019..[著者]
Masayoshi Onitsuka : Aggregation and misfolding of therapeutic antibodies in bioprocessing, The 57th Annual Meeting of the Biophysical Society of Japan, Symposium: The Quality of Proteins Multiple Approaches for Protein Evaluation, Sep. 2019..[著者]
Masayoshi Onitsuka, Shimadu AtsukoandIshikawa Shutaro : Reduced aggregation and altered N-glycosylation status of recombinant IgG1 in perfusion mammalian cell culture, 第20回日本蛋白質科学会年会, July 2020..[著者]
Masayoshi OnitsukaandShimazu Atsuko : Benchmark Study of Commercially Available Serum-free Media for CHO Cell Culture, The 33rd Annual and International Meeting of Japanese Association for Animal Cell Technology (JAACT), Fuchu, Japan, Nov. 2020..[著者]
Hirata Yuika, Amou Hiroe, Shimazu AtsukoandMasayoshi Onitsuka : Rapid Identification of Production Enhancer Gene (PEG) for Recombinant Antibody Production in CHO cells, The 33rd Annual and International Meeting of Japanese Association for Animal Cell Technology (JAACT), Fuchu, Japan, Nov. 2020..[著者]
SATOH Kohta, Amou Hiroe, Shimazu AtsukoandMasayoshi Onitsuka : Improved Terminal Galactosylation of Recombinant Antibody by Extracellular Glycosylation Reaction, The 33rd Annual and International Meeting of Japanese Association for Animal Cell Technology (JAACT), (巻), (号), (頁), Fuchu, Japan, Nov. 2020..[著者]
Kei Komatsu, Kento Kumon, Mayuno Arita, Masayoshi Onitsuka, Takeshi OmasaandMasafumi Yohda : Effect of the disulfide isomerase PDIa4 on the antibody production of Chinese hamster ovary cells., Journal of Bioscience and Bioengineering, Vol.130, No.6, 637-643, 2020..[著者]
Hirokazu Yagi, Saeko Yanaka, Rina Yogo, Akari Ikeda, Masayoshi Onitsuka, Toshio Yamazaki, Ttsuya Kato, Enoch Y. Park, Jun YokoyamaandKoichi Kato : Silkworm pupae function as efficient producers of recombinant glycoproteins with stable-isotope labeling., Biomolecules, Vol.10, No.11, 1482, 2020.---(Modified by 大家 隆弘 at 2021年1月25日(月) 20:24:04)
Kei Komatsu, Kento Kumon, Mayuno Arita, Masayoshi Onitsuka, Takeshi OmasaandMasafumi Yohda : Effect of the disulfide isomerase PDIa4 on the antibody production of Chinese hamster ovary cells., Journal of Bioscience and Bioengineering, Vol.130, No.6, 637-643, 2020.---(Modified by 大家 隆弘 at 2021年1月25日(月) 20:21:59)