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著作: Fogaça,E. Sandro/Melo,L. Robson/Pimenta,C. Daniel/[細井 和雄]/Juliano,Luiz/Juliano,A. Maria/Difference in substrate and inhibitor sequence specificity of human, mouse and rat tissue kallikreins/[The Biochemical Journal]

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EID
98192
EOID
673296
Map
0
LastModified
2012年12月20日(木) 11:04:10
Operator
松井 栄里
Avail
TRUE
Censor
0
Owner
細井 和雄
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Fogaça,E. Sandro
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Melo,L. Robson
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Pimenta,C. Daniel
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 細井 和雄
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Juliano,Luiz
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Juliano,A. Maria
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Difference in substrate and inhibitor sequence specificity of human, mouse and rat tissue kallikreins

副題 任意
要約 任意

(英) The kininogenase activities of mouse (mK1), rat (rK1) and human (hK1) tissue kallikreins were assayed with the bradykinin-containing synthetic peptides Abz-MTEMARRPPGFSPFRSVTVQNH2 (where Abz stands for o-aminobenzoyl) and Abz-MTSVIRRPPGFSPFRAPRV-NH2, which correspond to fragments Met374-Gln393 and Met375-Val393 of mouse and rat LMWKs (low-molecular-mass kininogens) with the addition of Abz. Bradykinin was released from these peptides by the mK1- and rK1-mediated hydrolysis of Arg-Arg and Arg-Ser (or Arg-Ala) peptide bonds. However, owing to preferential hydrolysis of Phe-Arg compared with the Arg-Ala bond in the peptide derived from rat LMWK, hK1 released bradykinin only from the mouse LMWK fragment and preferentially released des-[Arg9]bradykinin from the rat LMWK fragment (Abz-MTSVIRRPPGFSPFRAPRV-NH2). The formation of these hydrolysis products was examined in more detail by determining the kinetic parameters for the hydrolysis of synthetic, internally quenched fluorescent peptides containing six N- or C-terminal amino acids of bradykinin added to the five downstream or upstream residues of mouse and rat kininogens respectively. One of these peptides, Abz-GFSPFRAPRVQ-EDDnp (where EDDnp stands for ethylenediamine 2,4-dinitrophenyl), was preferentially hydrolysed at the Phe-Arg bond, confirming the potential des-[Arg9]bradykinin-releasing activity of hK1 on rat kininogen. The proline residue that is two residues upstream of bradykinin in rat kininogen is, in part, responsible for this pattern of hydrolysis, since the peptide Abz-GFSPFRASRVQ-EDDnp was preferentially cleaved at the Arg-Ala bond by hK1. Since this peptidase accepts the arginine or phenylalanine residue at its S1 subsite, this preference seems to be determined by the prime site of the substrates. These findings also suggested that the effects observed in rats overexpressing hK1 should consider the activation of B1 receptors by des-[Arg9]bradykinin. For further comparison, two short internally quenched fluorescent peptides that bind to hK1 with affinity in the nM range and some inhibitors described previously for hK1 were also assayed with mK1 and rK1.

キーワード 推奨
  1. (英) Amino Acid Sequence
  2. (英) Animals
  3. (英) Bradykinin
  4. (英) Enzyme Inhibitors
  5. (英) Female
  6. (英) Fluorescent Dyes
  7. (英) Humans
  8. (英) Hydrolysis
  9. (英) Kinins
  10. (英) Mice
  11. (英) Mice, Inbred ICR
  12. (英) Molecular Sequence Data
  13. (英) Peptide Fragments
  14. (英) Peptides
  15. (英) Rats
  16. (英) Substrate Specificity
  17. (英) Tissue Kallikreins
発行所 推奨
誌名 必須 The Biochemical Journal([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)
ISSN 任意 1470-8728
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 380
必須 Pt3
必須 775 781
都市 任意
年月日 必須 2004年 6月 15日
URL 任意
DOI 任意 10.1042/BJ20031047    (→Scopusで検索)
PMID 任意 15040788    (→Scopusで検索)
NAID 任意
WOS 任意 000222338100021
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Biophysics, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Tres de Maio 100, São Paulo 04044-20, Brazil.

  2. (英) Article.PublicationTypeList.PublicationType: Comparative Study

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article

  4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  5. (英) OtherID: PMC1224235