徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.ゲノム機能研究センター(〜2008年3月31日/->組織[徳島大学.疾患プロテオゲノム研究センター])

1. 山本 武範

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2. (英) Tachikawa Aiko

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3. (英) Terauchi Satsuki

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4. 山下 菊治

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5. 片岡 正俊

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6. (英) Terada Hiroshi

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7. 篠原 康雄([徳島大学.先端酵素学研究所.基幹研究部門])

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(英) Multiple Effects of DiS-C3(5) on Mitochondrial Structure and Function

(英) 3,3'-Dipropyl-2,2'-thiadicarbocyanine iodide [DiS-C(3)(5)], often used as a tracer dye to assess the mitochondrial membrane potential, was investigated in detail regarding its effects on the structure and function of isolated mitochondria. As reported previously, DiS-C(3)(5) had an inhibitory effect on NADH-driven mitochondrial electron transfer. On the contrary, in the presence of inorganic phosphate, DiS-C(3)(5) showed dose-dependent biphasic effects on mitochondria energized by succinate. At higher concentrations, such as 50 micro m, DiS-C(3)(5) accelerated mitochondrial oxygen consumption. Measurements of the permeability of DiS-C(3)(5)-treated mitochondrial membranes to poly(ethylene glycol) and analysis of mitochondrial configuration by transmission electron microscopy revealed that the accelerating effect of DiS-C(3)(5) on mitochondrial oxygen consumption reflects the induction of the mitochondrial permeability transition (PT). When the mitochondrial PT was induced by DiS-C(3)(5), release of mitochondrial cytochrome c was observed, as in the case of the PT induced by Ca(2+). On the contrary, at a low concentration such as 5 micro m, DiS-C(3)(5) showed an inhibitory effect on the latent oxygen consumption by mitochondria. This effect was shown to reflect inhibition of the PT induced by a low concentration of Ca(2+). Furthermore, in the absence of inorganic phosphate, DiS-C(3)(5) caused mitochondrial swelling. Under this condition, DiS-C(3)(5) caused changes in the membrane status of the mitochondria, but did not induce a release of mitochondrial cytochrome c.

1. (英) cyanine dye
2. (英) cytochrome c
3. (英) DiS-C-3(5)
4. (英) mitochondria
5. (英) permeability transition
6. (英) PERMEABILITY TRANSITION PORE
7. (英) CYTOCHROME-C RELEASE
8. (英) CYANINE DYE
9. (英) OXIDATIVE-PHOSPHORYLATION
10. (英) CATIONIC UNCOUPLER
11. (英) PHOSPHATE
12. (英) MECHANISMS
13. (英) DIS-C3-(5)
14. (英) TRANSPORT
15. (英) MEMBRANE

1. (英) Article.Affiliation: Institute for Genome Research, Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't