著作: Lu Guangming/[清水 一郎]/Cui Xuezhi/Itonaga Mina/Tamaki Katsuyoshi/Fukuno Hiroshi/Inoue Hiroshi/[本田 浩仁]/[伊東 進]/Interferon-α enhances biological defense activities against oxidative stress in cultured rat hepa-tocytes and hepatic stellate cells/[The Journal of Medical Investigation : JMI]
(英) Interferon-α enhances biological defense activities against oxidative stress in cultured rat hepa-tocytes and hepatic stellate cells
(英) Oxidative stress has been implicated as a cause of hepatic fibrosis, and hepatic stellate cells (HSCs), which are the most important collagen-producing cell types, have been reported to be activated by lipid peroxidation products. Antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GPx) provide a defense system that plays a critical role in protecting the cell from free radical damage, particularly lipid peroxidation. To elucidate the antioxidant activity of interferon-alpha (IFN-alpha), the effects of IFN-alpha on rat hepatocytes undergoing oxidative stress and HSCs in primary culture as well as isolated rat liver mitochondria were examined. IFN-alpha was observed to dose-dependently increase the immunoreactive protein levels of copper, zinc-and manganese-dependent SOD as well as the enzyme activities of GPx, and decrease the lipid peroxidation product levels and oxidative burst both in stressed hepatocytes and activated HSCs; GPx activities, however, were not detected in the latter cells. IFN-alpha also inhibited HSC activation and lipid peroxidation in liver mitochondria. These findings suggest that IFN-alpha may enhance biological defense activities against oxidative stress and function as a potent fibrosuppressant by protecting hepatocytes and hepatic stellate cells from lipid peroxidation in vivo.
The Journal of Medical Investigation : JMI([徳島大学.医学部])
|年月日||必須||2002年 8月 初日|