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著作: Kurihara, Kinji/[細井 和雄]/Kodama, Akiko/Ueha, Takao/A new electrophoretic variant of a subunit of Na+,K+-ATPase from the submandibular gland of rats/[Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology]

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EID
96153
EOID
711557
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2013年9月11日(水) 21:08:28
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大家 隆弘
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細井 和雄
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
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著者 必須
  1. (英) Kurihara, Kinji
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 細井 和雄
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Kodama, Akiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Ueha, Takao
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) A new electrophoretic variant of a subunit of Na+,K+-ATPase from the submandibular gland of rats

副題 任意
要約 任意

(英) The alpha catalytic subunits of Na+/K(+)-ATPase were isolated from the kidney and brain of rats (alpha 1 and alpha 2, respectively). The antisera raised against these subunits were used as probes to analyze the isoform of catalytic subunits of Na+/K(+)-ATPase in various tissues of rats. Of 27 rat tissues examined, most had a catalytic subunit identical to alpha 1 but some, such as the nervous and muscle tissues, had both alpha 1 and alpha 2 isoforms as judged by their reactivities to antisera and their electrophoretic mobility. We found that the submandibular gland contained a new electrophoretic variant of immunoreactive alpha subunit (designated alpha(S) in this report) in addition to alpha 1 identical to those found in kidney and brain. The new variant, alpha(S), strongly cross-reacted with anti-alpha 1 antiserum, but to a lesser extent with anti-alpha 2 antiserum. The alpha(S) had a molecular mass which was found to be slightly less (approx. 90 kDa) than brain and kidney alpha 1. We examined whether or not the alpha(S) is formed by proteolytic cleavage of alpha subunits during preparation and concluded that this is not the case. The alpha(S) reacted with [gamma-32P]ATP, resulting in the formation of radioactive alpha subunit which was stabilized by 2 mM ouabain but which was labile in the presence of 70 mM potassium chloride. Since N-terminal amino acid sequence of alpha(S) protein [G()DKY()PAAVS] corresponds exactly and uniquely with the sequence of the alpha 1 chain between residues 1 and 11, it is very probable that alpha(S) protein originated from alpha 1 protein following the post-translational processing.

キーワード 推奨
  1. (英) Amino Acid Sequence
  2. (英) Animals
  3. (英) Blotting, Western
  4. (英) Brain
  5. (英) Genetic Variation
  6. (英) Immune Sera
  7. (英) Isoenzymes
  8. (英) Kidney
  9. (英) Macromolecular Substances
  10. (英) Male
  11. (英) Microsomes
  12. (英) Molecular Sequence Data
  13. (英) Organ Specificity
  14. (英) Rats
  15. (英) Rats, Inbred Strains
  16. (英) Sequence Homology, Nucleic Acid
  17. (英) Sodium-Potassium-Exchanging ATPase
  18. (英) Submandibular Gland
発行所 推奨
誌名 必須 Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology([Elsevier Science])
(pISSN: 0167-4838)
ISSN 任意 0167-4838
ISSN: 0167-4838 (pISSN: 0167-4838)
Title: Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology
Publisher: Elsevier BV
 (Scopus  (CrossRef (Scopus information is found. [need login])
必須 1039
必須 2
必須 234 240
都市 任意
年月日 必須 1990年 6月 19日
URL 任意
DOI 任意 10.1016/0167-4838(90)90191-H    (→Scopusで検索)
PMID 任意 2163680    (→Scopusで検索)
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  1. (英) Article.Affiliation: Department of Oral Physiology, Meikai University, School of Dentistry, Saitama, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Comparative Study

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article

  4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't