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著作: Kurabuchi, Shingo/[細井 和雄]/Gresik, W Edward/Developmental and androgenic regulation of the immunocytochemical distribution of mK1, a true tissue kallikrein, in the granular convoluted tubule of the mouse submandibular gland/[The Journal of Histochemistry and Cytochemistry]

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EID
96027
EOID
678933
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0
LastModified
2013年2月18日(月) 10:27:27
Operator
松井 栄里
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Owner
細井 和雄
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 徳島大学.歯学部.歯学科.基礎口腔科学講座.口腔分子生理学
著者 必須
  1. (英) Kurabuchi, Shingo
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 細井 和雄
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Gresik, W Edward
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Developmental and androgenic regulation of the immunocytochemical distribution of mK1, a true tissue kallikrein, in the granular convoluted tubule of the mouse submandibular gland

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要約 任意

(英) The action of androgens on the immunocytochemical distribution of mK1, a true tissue kallikrein, was examined in the submandibular gland (SMG) of developing and adult mice by indirect enzyme-labeled and immunogold-labeled antibody methods for light and electron microscopy, respectively. In both sexes at 3 weeks of age, essentially all of the immature granular convoluted tubule (GCT) cells were uniformly immunostained. At 4 weeks of age (the onset of puberty), morphological differences between the two sexes appeared in the GCTs, in which some cells became immunonegative. Thereafter, the immunonegative GCT cells became more abundant in the SMG of males than of females and considerable intercellular variation in staining intensity for mK1 was seen, especially in males. A few slender GCT cells with strong immunoreactivity appeared in GCT segments only in males. Castration of males resulted in an increase in the number of immunopositive GCT cells, whereas administration of dihydrotestosterone (DHT) decreased the number of immunopositive GCT cells in the SMGs of both sexes. Slender GCT cells immunoreactive for mK1 were seen in females treated with DHT for 6 days. However, there were no immunostained slender GCT cells in female SMGs after injection of DHT for 2 weeks. Immunoelectron microscopy disclosed this type of cell in male SMGs, which closely resembles immature GCT cells of prepubertal mice, with a few small secretory granules uniformly labeled with gold particles, a sparse Golgi apparatus and RER, and basal infoldings. In mature male SMGs and in SMGs of DHT-treated females and castrated males, typical GCT cells had a well-developed Golgi apparatus and a net-like RER but few to no basal infoldings, whereas in the female gland equivalent cells had moderately developed RER and some basal infoldings. These results suggest that mK1 is one of the enzymes characteristically present in immature GCT cells and that its synthesis is inhibited in part by androgens, resulting in decreased numbers of immunopositive cells.

キーワード 推奨
  1. (英) Aging
  2. (英) Animals
  3. (英) Castration
  4. (英) Dihydrotestosterone
  5. (英) Female
  6. (英) Immunohistochemistry
  7. (英) Male
  8. (英) Mice
  9. (英) Mice, Inbred ICR
  10. (英) Sex Factors
  11. (英) Submandibular Gland
  12. (英) Tissue Kallikreins
発行所 推奨
誌名 必須 The Journal of Histochemistry and Cytochemistry(The Histochemical Society)
(pISSN: 0022-1554, eISSN: 1551-5044)
ISSN 任意 0022-1554
ISSN: 0022-1554 (pISSN: 0022-1554, eISSN: 1551-5044)
Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Title(ISO): J Histochem Cytochem
Publisher: Histochemical Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 50
必須 2
必須 135 145
都市 任意
年月日 必須 2002年 2月 初日
URL 任意
DOI 任意 10.1177/002215540205000202    (→Scopusで検索)
PMID 任意 11799133    (→Scopusで検索)
NAID 任意
WOS 任意 000173549200002
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Cell Biology and Anatomical Sciences, The City University of New York Medical School, New York 10031, USA.

  2. (英) Article.PublicationTypeList.PublicationType: Comparative Study

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article

  4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  5. (英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S.