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著作: Kurabuchi Shingo/[細井 和雄]/Gresik W Edward/Androgen regulation of the cellular distribution of the true tissue kallikrein mK1 in the submandibular gland of the mouse/[The Journal of Histochemistry and Cytochemistry]

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EID
95408
EOID
732259
Map
0
LastModified
2014年4月30日(水) 14:28:39
Operator
松井 栄里
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TRUE
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Owner
細井 和雄
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 徳島大学.歯学部.歯学科
著者 必須
  1. (英) Kurabuchi Shingo / (読) くらぶち しんご
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 細井 和雄
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Gresik W Edward
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Androgen regulation of the cellular distribution of the true tissue kallikrein mK1 in the submandibular gland of the mouse

副題 任意
要約 任意

(英) The kallikrein gene family encodes for at least four different proteases in the mouse submandibular gland (SMG): mK1 (true tissue kallikrein), mK9, mK13, and mK22. These enzymes and many other biologically active proteins are synthesized by the granular convoluted tubule (GCT), a specialized segment of the SMG duct system. The GCT is under multihormonal regulation by androgens, thyroid hormones, and adrenocortical hormones. Androgens suppress synthesis of mK1 in the SMG but enhance expression of the other three kallikreins. We prepared an antibody with limited immunoreactivity for mK1 and used it to examine the effects of androgen status on the distribution of this isozyme in the SMGs of developing and mature mice by immunoperoxidase staining for the light microscope and immunogold labeling for the electron microscope. In prepubertal mice, every immature GCT cell contains mK1, confined to an accumulation of small granules in the subluminal cytoplasm. In mature mice, not every GCT cell contains mK1, and in those cells that do there is considerable intergranular variation in the intensity of staining for mK1. GCT cells containing mK1 are much more abundant in the glands of females than of males, resulting in a peculiar sexually dimorphic mosaic distribution of this isozyme in the mature SMG. Castration of adult males increases the number of GCT cells expressing mK1. Administration of androgen to intact or castrated males or to intact females reduces the number of cells staining for mK1. In all cases, immunogold labeling for mK1 is confined to secretory granules. No fine structural differences were noted between cells that were positively or negatively stained for mK1. Therefore, although GCT cells appear to be composed of a uniform population of cells on the basis of morphology alone, they are not homogeneous in their content of secretory proteins. These results indicate that androgen regulation of GCT cells is more complex than has been appreciated to date.

キーワード 推奨
  1. (英) Androgens
  2. (英) Animals
  3. (英) Antibody Specificity
  4. (英) Female
  5. (英) Immunoenzyme Techniques
  6. (英) Isoenzymes
  7. (英) Male
  8. (英) Mice
  9. (英) Phenotype
  10. (英) Sex Characteristics
  11. (英) Sexual Maturation
  12. (英) Submandibular Gland
  13. (英) Tissue Distribution
  14. (英) Tissue Kallikreins
発行所 推奨
誌名 必須 The Journal of Histochemistry and Cytochemistry(The Histochemical Society)
(pISSN: 0022-1554, eISSN: 1551-5044)
ISSN 任意 0022-1554
ISSN: 0022-1554 (pISSN: 0022-1554, eISSN: 1551-5044)
Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Title(ISO): J Histochem Cytochem
Publisher: Histochemical Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 49
必須 6
必須 801 802
都市 任意
年月日 必須 2001年 6月 初日
URL 任意
DOI 任意 10.1177/002215540104900618    (→Scopusで検索)
PMID 任意 11373331    (→Scopusで検索)
NAID 任意
WOS 任意 000169233300018
Scopus 任意 2-s2.0-0034971585
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.PublicationTypeList.PublicationType: Journal Article