著作: [檜枝 光太郎]/Hirono Taisuke/Azami Akira/[鈴木 雅雄]/[古澤 佳也]/[前澤 博]/[宇佐美 徳子]/[横谷 明徳]/[小林 克己]/Single- and double-strand breaks in pBR322 plasmid DNA by monochromatic X-rays on and off thr K-absorption peak of phosphorus./[International Journal of Radiation Biology]
(英) Single- and double-strand breaks in pBR322 plasmid DNA by monochromatic X-rays on and off thr K-absorption peak of phosphorus.
(英) Using a synchrotron irradiation system pBR322 plasmid DNA was irradiated under vacuum by monochromatic X-rays having five specific photon energies (2.147, 2.153, 2.159, 2.168 and 2.199 keV) both on and off the K-absorption peak (2.153 keV) of phosphorus. The single- and double-strand breaks (ssb and dsb) were measured as conversions of the closed circular form of DNA (form I) to open circular (form II) and linear (form III) forms respectively. Exposures to induce one strand break per molecule were lowest at the peak (2.153 keV), and highest at 2.147 keV; the ratios were 2.7 for ssb and 3.0 for dsb. The exposures for dsb were 21-26 times higher than those for ssb. When the exposures were converted to absorbed doses in grays the absorbed doses per ssb were almost independent of photon energy. This result indicates that a certain absorbed dose was necessary to induce a ssb, regardless of whether photons were absorbed by the K-shell of phosphorus or by other shells, or by other atoms. However, the absorbed dose per dsb at 2.147 keV was 1.17 times higher than that averaged over four X-ray energies above 2-153 keV, indicating that the K-shell absorption, and the subsequent Auger event, efficiently induce dsb. The results are also discussed concerning the number of photo-absorptions of the constituent atoms per DNA strand break.
|発行所||推奨||Taylor & Francis|
International Journal of Radiation Biology([Taylor & Francis])
|年月日||必須||1996年 10月 末日|