著作: Kariya Kimio/[山内 あい子]/Sasaki T/Regional distribution and characterization of kinin in the CNS of the rat/[Journal of Neurochemistry]
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種別 | 必須 | 学術論文(審査論文) | |||||||||||||||||||||||||||
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言語 | 必須 | 英語 | |||||||||||||||||||||||||||
招待 | 推奨 | ||||||||||||||||||||||||||||
審査 | 推奨 | ||||||||||||||||||||||||||||
カテゴリ | 推奨 | ||||||||||||||||||||||||||||
共著種別 | 推奨 | ||||||||||||||||||||||||||||
学究種別 | 推奨 | ||||||||||||||||||||||||||||
組織 | 推奨 | ||||||||||||||||||||||||||||
著者 | 必須 |
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題名 | 必須 |
(英) Regional distribution and characterization of kinin in the CNS of the rat |
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副題 | 任意 | ||||||||||||||||||||||||||||
要約 | 任意 |
(英) The distribution of kinin in the CNS of the rat, which was extracted with n-butanol from an acidified homogenate, was determined using a bradykinin (BK) radioimmunoassay system. The immunoreactive kinin was widely distributed throughout the brain. The highest content was found in the pituitary gland (4,135 fmol BK Eq/g), followed by the medulla oblongata (912 fmol/g), cerebellum (549 fmol/g), and cortex (512 fmol/g). The kinin in the posterior pituitary was concentrated 4.5 times as much as in the anterior lobe. Serial dilution of brain extracts produced binding curves parallel to the standard radioimmunoassay curve. The purified brain kinin comigrated with authentic BK during CM-cellulose chromatography and Sephadex LH-20 gel chromatography. Its molecular weight was estimated to be 1,127 +/- 45 by gel filtration, which coincides well with that of BK. Chymotrypsin degraded the extracted kinin and authentic BK, but trypsin did not. These data demonstrate that a peptide indistinguishable from BK exists in the rat brain. Furthermore, pituitary kinin was separated into BK (87%), Lys-BK (10%), and Met-Lys-BK (3%), using reverse phase HPLC. |
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キーワード | 推奨 | ||||||||||||||||||||||||||||
発行所 | 推奨 | ||||||||||||||||||||||||||||
誌名 | 必須 |
Journal of Neurochemistry([The International Society for Neurochemistry])
(pISSN: 0022-3042, eISSN: 1471-4159)
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巻 | 必須 | 44 | |||||||||||||||||||||||||||
号 | 必須 | 6 | |||||||||||||||||||||||||||
頁 | 必須 | 1892 1897 | |||||||||||||||||||||||||||
都市 | 任意 | ||||||||||||||||||||||||||||
年月日 | 必須 | 1985年 6月 初日 | |||||||||||||||||||||||||||
URL | 任意 | ||||||||||||||||||||||||||||
DOI | 任意 | 10.1111/j.1471-4159.1985.tb07185.x (→Scopusで検索) | |||||||||||||||||||||||||||
PMID | 任意 | 3989568 (→Scopusで検索) | |||||||||||||||||||||||||||
CRID | 任意 | ||||||||||||||||||||||||||||
WOS | 任意 | ||||||||||||||||||||||||||||
Scopus | 任意 | 2-s2.0-0021887659 | |||||||||||||||||||||||||||
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指導教員 | 推奨 | ||||||||||||||||||||||||||||
備考 | 任意 |
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