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著作: [北岡 栄一郎]/[里村 一人]/Hayashi Eiji/[工藤 景子]/Tobiume Satoru/[長山 勝]/Establishment and charcterization of chondrocyte cell lines from the costal cartilage of SV40 large T antigen transgenic mice/[Journal of Cellular Biochemistry]

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EID
88274
EOID
730645
Map
0
LastModified
2014年4月15日(火) 14:03:35
Operator
松井 栄里
Avail
TRUE
Censor
0
Owner
工藤 景子
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.顎口腔再建医学講座(2004年4月1日〜)
著者 必須
  1. 北岡 栄一郎
    役割 任意 共著
    貢献度 任意
    学籍番号 推奨
  2. 里村 一人
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Hayashi Eiji
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 工藤 景子([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.口腔外科学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Tobiume Satoru
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. 長山 勝
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Establishment and charcterization of chondrocyte cell lines from the costal cartilage of SV40 large T antigen transgenic mice

副題 任意
要約 任意

(英) Complete understanding of the physiology and pathology of the cartilage is essential to establish treatments for a variety of cartilage disorders and defects such as rheumatoid arthritis, congenital malformations, and tumors of cartilage. Although synthetic materials have been used in many cases, they possess inherent problems including wear of the materials and low mechanical strength. Autograft has been considered very effective to overcome these problems. However, the limitation of the transplant volume is a major problem in autograft to be overcome. The costal cartilage is the most serious candidate for donor site transplantation, since it is the largest permanent hyaline cartilage in the body. To investigate the possibility using the costal cartilage as a transplant source, we have established and characterized three mouse chondrocyte cell lines (MCC-2, MCC-5, and MCC-35) derived from the costal cartilage of 8-week-old male SV40 large T-antigen transgenic mice. At confluence, all the cell lines formed nodules that could be positively stained with alcian blue (pH 2.5). The size of nodules gradually increased during culturing time. After 2 and 6 weeks of culture, RT-PCR analysis demonstrated that all three cell lines expressed mRNA from the cartilage-specific genes for type II collagen, type XI collagen, aggrecan, and link protein. Furthermore, type X collagen expression was detected in MCC-5 and MCC-35 but not in MCC-2. Any phenotypic changes were not observed over 31 cell divisions. Immunocytochemistry showed further that MCC-2, MCC-5, and MCC-35 produced cartilage-specific proteins type II collagen and type XI collagen, while in addition MCC-5 and MCC-35 produced type X collagen. Treatment with 1alpha, 25-dihydroxyvitamin D(3) inhibited cell proliferation and differentiation of the three cell lines in a dose-dependent manner. These phenotypic characteristics have been found consistent with chondrocyte cell lines established from cartilage tissues other than costal cartilage. In conclusion, costal cartilage shows phenotypic similarities to other cartilages, i.e., articular cartilage and embryonic limbs, suggesting that costal cartilage may be very useful as the donor transplantation site for the treatment of cartilage disorders. Furthermore, the cell lines established in this study are also beneficial in basic research of cartilage physiology and pathology.

キーワード 推奨
  1. (英) Alkaline Phosphatase
  2. (英) Animals
  3. (英) Antigens, Polyomavirus Transforming
  4. (英) Biological Markers
  5. (英) Calcitriol
  6. (英) Cartilage
  7. (英) Cell Division
  8. (英) Cell Line
  9. (英) Chondrocytes
  10. (英) Collagen
  11. (英) Extracellular Matrix
  12. (英) Gene Expression
  13. (英) Male
  14. (英) Mice
  15. (英) Mice, Transgenic
発行所 推奨
誌名 必須 Journal of Cellular Biochemistry([Wiley-Liss, Inc.])
(pISSN: 0730-2312, eISSN: 1097-4644)
ISSN 任意 0730-2312
ISSN: 0730-2312 (pISSN: 0730-2312, eISSN: 1097-4644)
Title: Journal of cellular biochemistry
Title(ISO): J Cell Biochem
Publisher: Wiley
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 81
必須 4
必須 571 582
都市 任意
年月日 必須 2001年 0月 初日
URL 任意
DOI 任意 10.1002/jcb.1075    (→Scopusで検索)
PMID 任意 11329612    (→Scopusで検索)
NAID 任意
WOS 任意 000168462100001
Scopus 任意 2-s2.0-0035016352
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.PublicationTypeList.PublicationType: Journal Article