徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.歯学部.歯学科.基礎口腔科学講座.分子薬理学 (歯科薬理学講座)(〜2020年6月30日)

1. 水澤 典子([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])

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2. (英) Hasegawa, Tomoko / (日) 長谷川 朋子

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3. 大東 いずみ([徳島大学.先端酵素学研究所.重点研究部門])

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4. 小杉 知里

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5. 原田 永勝

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6. 板倉 光夫

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7. 吉本 勝彦

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(英) Differentiation Penotypes of Pancreatic Islet β- and α-cells are Closely Related with Homeotic Genes and a Group of Differentially Expressed Genes.

(英) To identify the genes that determine differentiation phenotypes, we compared gene expression of pancreatic islet beta- and alpha-cells, which are derived from the common precursor and secrete insulin and glucagon, respectively. The expression levels of homeotic genes including Hox genes known to determine region specificity in the antero-posterior (AP) body axis, tissue-specific homeobox genes, and other 8,734 genes were compared in a beta- and alpha-cell line of MIN6 and alpha TC1.6. The expression of homeotic genes were surveyed with reverse transcription-polymerase chain reaction (RT-PCR) using degenerate primers corresponding to invariant amino acid sequences within the homeodomain and subsequently with specific primers. Expression of Hoxc6, Hoxc9, Hoxc10, Pdx1, Cdx2, Gbx2, Pax4, and Hlxb9 genes in MIN6 was higher than those in alpha TC1.6, while expression of Hoxa2, Hoxa3, Hoxa5, Hoxa6, Hoxa7, Hoxa9, Hoxa10, Hoxa13, Hoxb3, Hoxb5, Hoxb6, Hoxb13, Hoxb8, and Brain4 genes in alpha TC1.6 was higher than those in MIN6. Out of 8,734 mouse genes screened with high-density mouse cDNA microarrays for MIN6- and alpha TC1.6-derived cDNA, 58 and 25 genes were differentially over- and under-expressed in MIN6, respectively. GLUTag, which is derived from a large bowel tumor and expresses the proglucagon gene, showed a comparatively similar expression profile to that of alpha TC1.6 in both homeotic and other genes analyzed in cDNA microarray. Our results are consistent with the interpretation that not only the tissue-specific homeotic genes, but also Hox genes are related to differentiation phenotypes of pancreatic beta- and alpha-cells rather than their regional specification of the body in vertebrates.

1. (英) Animals
2. (英) Blotting, Northern
3. 細胞分化(cell differentiation)
4. (英) Cell Line, Transformed
5. (英) Cell Line, Tumor
6. (英) DNA, Complementary
7. (英) Gene Expression Profiling
8. (英) Genes, Homeobox
9. (英) Islets of Langerhans
10. (英) Mice
11. (英) NIH 3T3 Cells
12. (英) Oligonucleotide Array Sequence Analysis
13. (英) Phenotype
14. (英) RNA, Messenger
15. (英) Reverse Transcriptase Polymerase Chain Reaction

1. (英) Article.Affiliation: Department of Pharmacology, School of Dentistry, The University of Tokushima, Tokushima City, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Comparative Study

3. (英) Article.PublicationTypeList.PublicationType: Journal Article

4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't