徳島大学 教育・研究者情報データベース(EDB)

Education and Research Database (EDB), Tokushima University

徳島大学ウェブサイトへのリンク

著作: [松井 好人]/Matrix deposition of tryptophan-containing allelic variants of type IX collagen in developing human cartilage./[Matrix Biology]

ヘルプを読む

「著作」(著作(著書,論文,レター,国際会議など))は,研究業績にかかる著作(著書,論文,レター,国際会議など)を登録するテーブルです. (この情報が属するテーブルの詳細な定義を見る)

  • 項目名の部分にマウスカーソルを置いて少し待つと,項目の簡単な説明がツールチップ表示されます.

この情報をEDB閲覧画面で開く

EID
87013
EOID
667839
Map
0
LastModified
2012年10月27日(土) 16:18:10
Operator
大家 隆弘
Avail
TRUE
Censor
0
Owner
[研究部長]/[徳島大学.大学院ヘルスバイオサイエンス研究部]
Read
継承
Write
継承
Delete
継承
種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 徳島大学.大学院ヘルスバイオサイエンス研究部.再生修復医歯学部門.感覚運動系病態医学講座(2004年4月1日〜)
著者 必須
  1. 松井 好人
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Matrix deposition of tryptophan-containing allelic variants of type IX collagen in developing human cartilage.

副題 任意
要約 任意

(英) Genetic polymorphisms that encode a tryptophan (Trp) residue in the triple-helical domain of the alpha2 (Trp2) or alpha3 chain (Trp3) of human type IX collagen have been linked to risk of degenerative intervertebral disc disease. To determine whether these two allelic variants express protein that may affect the extracellular matrix of cartilage in vivo, we examined the properties of resident type IX collagen in an anonymous collection of embryonic and fetal human cartilage samples screened for Trp genotypes. No difference was found in the yield and electrophoretic properties of pepsin-solubilized type IX collagen between Trp2, Trp3 and non-Trp cartilage samples. On Western blot analysis, a polyclonal antiserum raised against a synthetic peptide matching the immediate Trp-containing sequence of the Trp3 allele reacted specifically with the alpha3(IX) chain prepared from Trp3 cartilage samples. Two-dimensional peptide mapping of type IX collagen in CNBr-digests of whole tissue gave indistinguishable fingerprints for Trp2, Trp3 and control tissues, including the yield of cross-linked peptides. Analysis of one cartilage sample that was homozygous for the Trp2 allele also gave a normal yield of collagen IX, including its alpha2 chain and a normal profile of cross-linked peptides. Together, the findings indicate that both Trp2 and Trp3 allelic products are incorporated into the cross-linked fibrillar network of developing human cartilage apparently normally. Any pathological consequences are likely, therefore, to be long-term and indirect rather than from overt misassembly of matrix.

キーワード 推奨
  1. (英) Alleles
  2. (英) Amino Acid Sequence
  3. (英) Base Sequence
  4. (英) Cartilage
  5. (英) Collagen Type IX
  6. (英) Embryo, Mammalian
  7. (英) Embryonic and Fetal Development
  8. (英) Extracellular Matrix
  9. (英) Gene Frequency
  10. (英) Genetic Variation
  11. (英) Humans
  12. (英) RNA, Messenger
  13. (英) Tryptophan
発行所 推奨
誌名 必須 Matrix Biology(International Society for Matrix Biology)
(pISSN: 0945-053X, eISSN: 1569-1802)
ISSN 任意 0945-053X
ISSN: 0945-053X (pISSN: 0945-053X, eISSN: 1569-1802)
Title: Matrix biology : journal of the International Society for Matrix Biology
Title(ISO): Matrix Biol
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 22
必須 2
必須 123 129
都市 任意
年月日 必須 2003年 4月 末日
URL 任意
DOI 任意 10.1016/S0945-053X(02)00102-6    (→Scopusで検索)
PMID 任意 12782139    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Orthopaedic Research Laboratories, Department of Orthopaedics and Sports Medicine, University of Washington, P.O. Box 356500, Seattle, WA 98195-6500, USA.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S.