著作: [中西 正]/[清水 洋利]/[細川 義隆]/[松尾 敬志]/An immunohistological study on cyclooxygenase-2 in human dental pulp./[Journal of Endodontics]
(英) An immunohistological study on cyclooxygenase-2 in human dental pulp.
(英) Characteristics of cyclooxygenase-2 (COX-2) expressing cells in human dental pulp were immunohistologically studied. Extirpated pulpal tissues from extracted teeth were examined to elucidate the localization and distribution of COX-2. Pulpal tissues were examined by the labeled streptavidin biotin method using specific mouse monoclonal antibodies for COX-2. Cell types of the COX-2 expressing cells were also investigated by the double stain technique using both monoclonal antibodies for CD68/macrophage and anti-COX-2. COX-2 expressing cells could be found in all of the inflamed pulps, and these cells were mostly distributed close to the area of accumulation of inflammatory cells. COX-2 was mainly expressed in fibroblasts rather than macrophages. In contrast, COX-2 expressing cells were scarcely found in the normal pulps. These findings indicate that pulpal fibroblasts, as well as macrophages, may participate in the production of prostaglandin through COX-2 expression in pulpal inflammation, and might be involved in the pathogenesis of irreversible pulpitis.
|発行所||推奨||The American Association of Endodontics|
Journal of Endodontics([The American Association of Endodontics])
|年月日||必須||2001年 6月 初日|