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著作: Aoyama Chieko/[山﨑 尚志]/[寺田 弘]/Ishidate Kozo/Structure and characterization of the genes for murine choline/ethanolamine kinase isozymes alpha and beta./[Journal of Lipid Research]

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EID
84799
EOID
701301
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0
LastModified
2013年8月12日(月) 15:55:38
Operator
大家 隆弘
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TRUE
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0
Owner
山﨑 尚志
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨 研究
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 薬品素材学講座(〜2006年3月31日)
著者 必須
  1. (英) Aoyama Chieko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 山﨑 尚志([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.薬物治療学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 寺田 弘([東京理科大学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Ishidate Kozo
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Structure and characterization of the genes for murine choline/ethanolamine kinase isozymes alpha and beta.

副題 任意
要約 任意

(英) Choline/ethanolamine kinase (CK/EK) is the first enzyme in phosphatidylcholine/phosphatidylethanolamine biosynthesis in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products so far were all shown to have EK activity also, indicating that both activities reside on the same protein. CK/EK in most animal cells exists as several isoforms, for two of which (alpha and beta) their cDNAs have been cloned from both the rat and mouse, and they are found to be separate gene products. The physiological significance for the existence of more than one CK/EK enzyme, however, remains to be clarified. In this study, we isolated mouse genes encoding both types of CK/EK isozyme and determined their entire structure. The 5'-flanking promoter regions were found to have quite different features from each other, indicating that their expression could be under distinct control. Comparison of the nucleotide sequence between the corresponding coding exons showed the best homology (75%) residing on exon VIII. A search of the database resulted in the possible existence of 17 different origins of eukaryotic CK and/or EK, each of which presumably contained the entire amino acid sequence. Multialignment of their putative amino acid sequences led to an identification of the novel consensus sequence possibly required for the expression of either CK or EK activity, which corresponded to the sequence within exons VII and VIII of CK/EK-alpha and -beta genes from the mouse. This sequence was localized in close proximity to the C-terminal region of the general (Brenner's) phosphotransferase concensus sequence which was also completely conserved in all of the putative eukaryotic CK/EK proteins. The results demonstrated that, while both CK/EK-alpha and -beta genes were composed of 11 major exons, the size of their genes was quite different: 40 kb for CK/EK-alpha, whereas it was only 3.5 kb for CK/EK-beta.

キーワード 推奨
  1. (英) Amino Acid Sequence
  2. (英) Animals
  3. (英) Base Sequence
  4. (英) Choline Kinase
  5. (英) Consensus Sequence
  6. (英) DNA
    データベース中に適合する可能性のある以下の情報を発見しました
    [キーワード] 酸化DNA傷害 (oxidative DNA damage)
    [キーワード] DNA (DNA)
    [キーワード] ミトコンドリアDNA (mitochondrial DNA)
    [キーワード] cDNA subtractive screening (cDNA subtractive screening)
  7. (英) DNA Primers
  8. (英) Exons
  9. (英) Humans
  10. (英) Isoenzymes
  11. (英) Mice
    データベース中に適合する可能性のある以下の情報を発見しました
    [キーワード] ノックアウトマウス (knockout mice)
  12. (英) Molecular Sequence Data
  13. (英) Phosphotransferases (Alcohol Group Acceptor)
  14. (英) Promoter Regions, Genetic
  15. (英) Rats
  16. (英) Sequence Homology, Amino Acid
発行所 推奨
誌名 必須 Journal of Lipid Research([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0022-2275, eISSN: 1539-7262)
ISSN 任意 0022-2275
ISSN: 0022-2275 (pISSN: 0022-2275, eISSN: 1539-7262)
Title: Journal of lipid research
Title(ISO): J. Lipid Res.
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 41
必須 3
必須 452 464
都市 任意
年月日 必須 2000年 3月 初日
URL 任意
DOI 任意
PMID 任意 10706593    (→Scopusで検索)
NAID 任意
WOS 任意 000086224900015
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Medical Research Institute, Tokyo Medical and Dental University, Chiyodaku, Tokyo 101-0062, USA.

  2. (英) Article.DataBankList.DataBank.DataBankName: GENBANK

  3. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB030615

  4. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB030616

  5. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB030617

  6. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB030618

  7. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB030619

  8. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB030620

  9. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB030621

  10. (英) Article.PublicationTypeList.PublicationType: Journal Article