著作: Daikoku Takiko/[篠原 康雄]/Shima Atsushi/[山﨑 尚志]/[寺田 弘]/Specific elevation of transcript levels of particular protein subtypes induced in brown adipose tissue by cold exposure./[Biochimica et Biophysica Acta (BBA) - Bioenergetics]
(英) Specific elevation of transcript levels of particular protein subtypes induced in brown adipose tissue by cold exposure.
(英) To understand the difference in metabolic flow in rat brown adipose tissue (BAT) from that in white adipose tissue (WAT) at the molecular level, we examined the steady-state transcript levels of 39 proteins in both adipose tissues with and without cold exposure by Northern blot analysis. In addition to the transcript levels of uncoupling protein isoforms, those of proteins involved in the transport and catabolism of fatty acids and glucose in BAT were elevated by cold exposure, suggesting the stimulation of utilization of fatty acids and glucose as fuels in BAT. As to these changes, the muscle-type subtypes were remarkable; and therefore, they were suggested to be responsible for the cold exposure-induced acceleration of energy expenditure in BAT. Furthermore, of the isoforms of beta-adrenergic receptor (beta-AR) and CCAAT enhancer binding protein (C/EBP), transcript levels of beta(1)-AR and C/EBPbeta in BAT were increased by the cold exposure. Possible roles of these proteins in energy metabolism in BAT were discussed.
Biochimica et Biophysica Acta (BBA) - Bioenergetics([Elsevier Science])
|年月日||必須||2000年 4月 21日|