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著作: [木戸 淳一]/Nishikawa S./Ishida H./[山下 菊治]/[北村 清一郎]/Kohri K./[永田 俊彦]/Identification of calprotein , a calcium binding leukocyte protein, In human dental calculus matrix/[Journal of Periodontal Research]

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EID
79650
EOID
848219
Map
0
LastModified
2017年2月23日(木) 10:46:12
Operator
三木 ちひろ
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TRUE
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Owner
木戸 淳一
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
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共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 木戸 淳一([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Nishikawa S.
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    貢献度 任意
    学籍番号 推奨
  3. (英) Ishida H.
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 山下 菊治
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    学籍番号 推奨
  5. 北村 清一郎
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    学籍番号 推奨
  6. (英) Kohri K.
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  7. 永田 俊彦
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    学籍番号 推奨
題名 必須

(英) Identification of calprotein , a calcium binding leukocyte protein, In human dental calculus matrix

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要約 任意

(英) Calprotectin is a calcium binding protein produced by leukocytes, macrophages and epithelial cells, and its levels in several tissues increase during infections and in many inflamed areas, suggesting that it may be an indicator of inflammatory activity. Osteopontin is a prominent phosphorylated glycoprotein in bone matrix, having calcium binding capacity. Recently, it has been reported that calprotectin and osteopontin are present in urinary stones (pathological mineralized masses in the body), and that these proteins may be involved in their formation. Dental calculus formed by mineralization of dental plaque is an inflammatory factor which may contribute to periodontal disease. It contains many organic components involved in mineralization. We recently found osteopontin molecules in human dental calculus and suggested that the components of its matrix may be similar to those of urinary stones. In this study, we investigated the presence of calprotectin in human dental calculus by immunohistochemical and immunoblotting analyses using a specific antibody for calprotectin. After fixation and demineralization of dental calculi adhered to tooth roots, sections embedded in paraffin were immunoreacted with the antibody for calprotectin and positive immunostaining for calprotectin was observed. Dental calculus proteins were then extracted with EDTA and separated by electrophoresis on 15% polyacrylamide gels. By immunoblotting analysis, 3 or 4 bands were observed at 11, 14.5, 22-25, 28 or 36.5 kDa and these patterns corresponded to those of calprotectin subunits. When non-immune rabbit serum was used instead of calprotectin-specific antibody as a negative control, no immunoreactivity was observed. These findings indicate that calprotectin is associated not only with antibacterial action but also with calcium binding capacity during dental calculus formation.

キーワード 推奨
  1. (英) Calcium-Binding Proteins
  2. (英) Decalcification Technique
  3. (英) Dental Calculus
  4. (英) Electrophoresis, Polyacrylamide Gel
  5. (英) Humans
  6. (英) Immunoblotting
  7. 免疫組織化学(immunohistochemistry)
  8. (英) Leukocyte L1 Antigen Complex
  9. (英) Neural Cell Adhesion Molecules
発行所 推奨
誌名 必須 Journal of Periodontal Research([Blackwell Publishing Ltd])
(pISSN: 0022-3484, eISSN: 1600-0765)
ISSN 任意 0022-3484
ISSN: 0022-3484 (pISSN: 0022-3484, eISSN: 1600-0765)
Title: Journal of periodontal research
Title(ISO): J Periodontal Res
Publisher: Blackwell
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 32
必須 4
必須 335 361
都市 任意
年月日 必須 1997年 5月 初日
URL 任意
DOI 任意 10.1111/j.1600-0765.1997.tb00545.x    (→Scopusで検索)
PMID 任意 9210089    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.PublicationTypeList.PublicationType: Journal Article