著作: [赤松 徹也]/Parvin, Nahid Most/Murdiastuti, Kwartarini/[小杉 知里]/[姚 陳娟]/Miki, Osamu/[金森 憲雄]/[細井 和雄]/Expression and localization of aquaporins, members of the water channel family, during development of the rat submandibular gland./[Pflügers Archiv : European Journal of Physiology]
(英) Expression and localization of aquaporins, members of the water channel family, during development of the rat submandibular gland.
(英) The expression and localization of aquaporins (AQP1-AQP5), members of the water channel family, in the developing rat submandibular gland were analysed using RT-PCR, Northern blotting and immunohistochemistry to explore their relation to the development of this salivary gland. RT-PCR analysis revealed unique expression patterns of each AQP. AQP1 was expressed constitutively during prenatal development, whereas the expression of AQP5 became more intense in the course of development from embryonic day 16.5 (E16) to E20. These expression patterns concurred with the results of Northern blot analysis. AQP3 and AQP4 mRNAs in the prenatal development were not detected in Northern blots, although they were detected by RT-PCR. During postnatal development, AQP5 and AQP1 mRNAs were expressed continuously, but no message for AQP3 or AQP4 was detected. AQP2 mRNA was not detected during either prenatal or postnatal development in this tissue. Immunohistochemical studies revealed that AQP5 was first localized at the apical membrane of proacinar cells at E18, and then became clearly distributed at the apical membrane of acinar cells in accordance with the differentiation and establishment of the mature acini. In addition, some vasculature also showed immunoreactivity for AQP5. AQP1 was immunolocalized in the blood vessels, including capillaries, of the gland throughout development. These observations suggest the existence of transcriptional regulation of rat AQP5, which is one of the most probable regulators of saliva production and secretion, during the establishment of the functional submandibular salivary gland.
Pflügers Archiv : European Journal of Physiology([Springer-Verlag])
|年月日||必須||2003年 6月 28日|