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著作: Honda, Soichi/[小杉 知里]/Yamada, Shozo/[佐野 壽昭]/[松本 俊夫]/[板倉 光夫]/[吉本 勝彦]/Human Pituitary Adenomas Infrequently Contain Inactivation of Retinoblastoma 1 Gene and Activation of Cyclin Dependent Kinase 4 Gene./[Endocrine Journal]

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EID
72276
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LastModified
2018年8月29日(水) 15:37:33
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吉本 勝彦
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 徳島大学.医学部
著者 必須
  1. (英) Honda, Soichi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 小杉 知里
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Yamada, Shozo
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 佐野 壽昭
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 松本 俊夫
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. 板倉 光夫
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. 吉本 勝彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Human Pituitary Adenomas Infrequently Contain Inactivation of Retinoblastoma 1 Gene and Activation of Cyclin Dependent Kinase 4 Gene.

副題 任意
要約 任意

(英) Components of cyclinD1/cyclin-dependent kinase 4 (CDK4)/p16INK4a/pRb pathway are the frequent target of many tumor types. We examined the role of retinoblastoma susceptibility gene (RB1) and the CDK4 gene in human pituitary tumorigenesis. For the RB1 gene, pRb expression and loss of heterozygosity (LOH) on 13q in pituitary adenomas were analysed. Immunostaining of pRb revealed lack of expression in 1 of 29 pituitary adenomas. In 4 of 31 pituitary adenomas, allelic imbalances including LOH of RB1 on 13q14 were detected. Three of 4 pituitary adenomas, in which one adenoma lacked pRb expression, had a common LOH region at least from D13S219 on 13q12.3-q13 to D13S265 on 13q31-32. Interphase fluorescence in situ hybridization with a probe of RB1 showed 2 copies of RB1 gene suggesting that mitotic recombination events, not deletion or chromosome loss, led to LOH in the 3 pituitary adenomas analyzed. All 27 exons, intron-exon boundaries, and essential promoter region of RB1 gene were then sequenced in genomic DNA from 4 pituitary adenomas with allelic imbalance on 13q14 including one adenoma without pRb expression and 3 adenomas with pRb expression. Any somatic mutations, insertions, or microdeletions in the RB1 gene were not detected in 4 pituitary adenomas. Methylation sensitive (MS)-polymerase chain reaction (PCR) and bisulfite sequencing analysis revealed hypomethylated status of CpG islands in the promoter region of the RB1 genes of 4 pituitary adenomas. In addition, activating mutations of CDK4 gene, which is a component of cyclinD1/CDK4/p16INK4a/pRb pathway, were not detected in 31 pituitary adenomas. Based on these results, it is concluded that somatic mutations of the RB1 gene or CDK4 gene do not appear to play a major role in pituitary tumorigenesis. This supports the presence of potential tumor suppressor gene(s) on 13q12.3-q13 to 13q31-32 in pituitary adenomas.

キーワード 推奨
  1. (英) Adenoma
  2. (英) Adult
  3. (英) Aged
  4. (英) Chromosomes, Human, Pair 13
  5. (英) Cyclin-Dependent Kinase 4
  6. (英) Cyclin-Dependent Kinases
  7. (英) DNA Methylation
  8. (英) Female
  9. (英) Gene Expression Regulation, Neoplastic
  10. (英) Genes, Retinoblastoma
  11. (英) Humans
  12. (英) Immunohistochemistry
  13. (英) In Situ Hybridization, Fluorescence
  14. (英) Loss of Heterozygosity
  15. (英) Male
  16. (英) Middle Aged
  17. (英) Mutation
  18. (英) Pituitary Neoplasms
  19. (英) Polymerase Chain Reaction
  20. (英) Promoter Regions, Genetic
  21. (英) Proto-Oncogene Proteins
発行所 推奨
誌名 必須 Endocrine Journal([社団法人 日本内分泌学会])
(pISSN: 0918-8959, eISSN: 1348-4540)
ISSN 任意 0918-8959
ISSN: 0918-8959 (pISSN: 0918-8959, eISSN: 1348-4540)
Title: Endocrine journal
Title(ISO): Endocr J
Supplier: 一般社団法人 日本内分泌学会
Publisher: Japan Endocrine Sociey/Nihon Naibunpi Gakkai
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 50
必須 3
必須 309 318
都市 任意
年月日 必須 2003年 3月 初日
URL 任意
DOI 任意 10.1507/endocrj.50.309    (→Scopusで検索)
PMID 任意 12940460    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
機関リポジトリ 111695
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Medicine and Bioregulatory Sciences, Graduate School of Medicine, The University of Tokushima, Tokushima 770-8503, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't