徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)

1. (英) Goto Kaho

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   貢献度	任意
   学籍番号	推奨

2. (英) Fukuda Jinichi

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   学籍番号	推奨

3. 羽地 達次

   役割	任意	共著
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   学籍番号	推奨

(英) Okadaic acid stimulates apoptosis through expression of Fas receptor and Fas ligand in human squamous cell carcinoma cells.

(英) Fas receptor is a member of a superfamily of receptors characterized by cysteine-rich motifs in the extracellular domain of the molecule. Binding of Fas ligand to Fas receptor leads to activation of the latter and the induction of intracellular signals that result in apoptotic cell death. In the present study, we used reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis to examine the expression of mRNAs and proteins of Fas receptor and Fas ligand in human oral squamous carcinoma SCC-25 cells treated with okadaic acid. The PCR product of Fas receptor mRNA was detected in the cells and a protein with an estimated molecular weight of 35,000 was also expressed in them. Expression of Fas receptor mRNA stimulated by okadaic acid was elevated in dose- and time-dependent manners as judged by semiquantitative RT-PCR analysis, with the maximum expression level at 50 nM and 8 h treatment. Fas ligand mRNA expression was also stimulated by okadaic acid in SCC-25 cells in dose- and time-dependent manners. Okadaic acid also stimulated the expression of Fas ligand protein in the cells. Okadaic acid in serum-free medium induced apoptosis in SCC-25 cells in a time-dependent manner up to 24 h as determined by nuclear condensation and fragmentation of chromatin and DNA ladder formation. The present results indicate that the expression of Fas receptor and Fas ligand is negatively regulated by a protein phosphatase(s) sensitive to okadaic acid and is involved in okadaic acid-induced apoptosis in SCC-25 cells. Our results also suggest that Fas receptor and Fas ligand system might regulate apoptosis in SCC-25 cells in an autocrine fashion.

1. (英) Antigens, CD95
2. アポトーシス(apoptosis)
3. (英) Blotting, Western
4. (英) Carcinoma, Squamous Cell
5. (英) Culture Media, Serum-Free
6. (英) Dose-Response Relationship, Drug
7. (英) Electrophoresis, Polyacrylamide Gel
8. (英) Enzyme Inhibitors
9. (英) Fas Ligand Protein
10. (英) Humans
11. (英) Membrane Glycoproteins
12. (英) Neoplasm Proteins
13. (英) Okadaic Acid
14. (英) RNA, Messenger
15. (英) Reverse Transcriptase Polymerase Chain Reaction
16. (英) Tongue Neoplasms
17. (英) Tumor Cells, Cultured

1. (英) Article.PublicationTypeList.PublicationType: Journal Article

2. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't