徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)

1. (英) Morimoto Yasuhiro

   役割	任意
   貢献度	任意
   学籍番号	推奨

2. (英) Kito Shinji

   役割	任意
   貢献度	任意
   学籍番号	推奨

3. (英) Ohba Takeshi

   役割	任意
   貢献度	任意
   学籍番号	推奨

4. 森本 景之

   役割	任意	共著
   貢献度	任意
   学籍番号	推奨

5. 岡村 裕彦

   役割	任意
   貢献度	任意
   学籍番号	推奨

6. 羽地 達次

   役割	任意
   貢献度	任意
   学籍番号	推奨

(英) Alteration of argyrophilic nucleolar organizer region associated (Ag-NOR) proteins in apoptosis-induced human salivary gland cells and human squamous carcinoma cells.

(英) The level of argyrophilic nucleolar organizer regions (AgNORs) and AgNOR-associated proteins (Ag-NOR proteins) varies with cell activity, including ribosomal biogenesis occurring in proliferating cells. Proteins associated with some AgNORs are detected by a specific silver staining. To investigate a possible relationship between apoptosis and the AgNORs or Ag-NOR proteins, we examined the changes of AgNORs and Ag-NOR proteins during apoptosis in a human salivary gland cell line, HSG cells, and a human oral squamous carcinoma cell line, SCC-25 cells. Apoptosis was induced by treatment of HSG and SCC-25 cells with okadaic acid. Proteins prepared from HSG and SCC-25 cells treated with varying concentrations of okadaic acid (OA) were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by transferring to transfer membranes and staining for Ag-NOR proteins by modified Western blot analysis. Four major bands (110 kDa, 43 kDa, 39kDa, and 37 kDa) were detected in the proteins obtained from the control cells. The level of the 110-kDa protein decreased in the proteins prepared from OA-induced apoptotic cells; however, the reaction intensity of the other three bands was changed in apoptotic cells. An additional band of an 80-kDa Ag-NOR protein appeared and increased in the apoptotic cells. Cellular fractionation of HSG cells and SCC-25 cells was done with or without apoptotic induction. An 80-kDa Ag-NOR protein was detected in the nuclear fraction prepared from the apoptotic cells, while the 110-kDa protein decreased in the nuclear fraction of these cells. The 110-kDa Ag-NOR protein may be nucleolin (C23) as deduced from its AgNOR staining features, including molecular weight. The 80-kDa protein may be the cleavage product of the 110-kDa protein. In the cell-free apoptotic system, in which intact nuclei of HSG cells were incubated with the cytosol fraction of apoptotic HSG and SCC-25 cells, the 80-kDa Ag-NOR protein was detected in nuclei incubated with the cytosol fraction of apoptotic cells, while the level of the 110-kDa protein decreased. The changes of Ag-NOR proteins in nuclei prepared from SCC-25 cells incubated with cytosol fractions prepared from HSG and SCC-25 cells were identical to those of the HSG cells. The alternation of AgNORs in apoptosis-induced HSG cells was also examined using double staining with Hoechst 33342 and silver nitrate. Hoechst staining revealed typical apoptotic nuclei, which exhibited highly fluorescent condensed chromatin in OA-treated HSG cells. Silver grains representing AgNORs were not detected in the cells undergoing apoptosis. The dual-imposition view confirmed that AgNORs, which are visible as dots in nucleoli in the control cells, disappeared from the apoptotic nuclei of HSG cells. Our results indicate that the 110-kDa nucleolar Ag-NOR protein is associated with apoptosis and is cleaved during apoptosis.

1. (英) Antigens
2. (英) Antigens, Nuclear
3. アポトーシス(apoptosis)
4. (英) Benzimidazoles
5. (英) Blotting, Western
6. (英) Carcinoma, Squamous Cell
7. (英) Cell Line
8. (英) Cell Nucleus
9. (英) Chromatin
10. (英) Coloring Agents
11. (英) Cytosol
12. (英) Electrophoresis, Polyacrylamide Gel
13. (英) Enzyme Inhibitors
14. (英) Fluorescent Dyes
15. (英) Humans
16. 分子量(molecular weight)
17. (英) Mouth Neoplasms
18. (英) Nuclear Proteins
19. (英) Nucleolus Organizer Region
20. (英) Okadaic Acid
21. (英) Phosphoproteins
22. (英) RNA-Binding Proteins
23. 唾液腺(salivary glands/->キーワード[唾液腺])
24. (英) Silver
25. (英) Silver Nitrate
26. (英) Tumor Cells, Cultured

1. (英) Article.PublicationTypeList.PublicationType: Journal Article

2. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't