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著作: Morimoto Yasuhiro/Kito Shinji/Ohba Takeshi/[森本 景之]/[岡村 裕彦]/[羽地 達次]/Alteration of argyrophilic nucleolar organizer region associated (Ag-NOR) proteins in apoptosis-induced human salivary gland cells and human squamous carcinoma cells./[Journal of Oral Pathology & Medicine]

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EID
70917
EOID
887165
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LastModified
2018年2月7日(水) 11:31:22
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三木 ちひろ
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[副研究部長]/[徳島大学.大学院医歯薬学研究部]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨 研究
共著種別 推奨 国内共著(徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
学究種別 推奨
組織 推奨
  1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)
著者 必須
  1. (英) Morimoto Yasuhiro
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Kito Shinji
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Ohba Takeshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 森本 景之
    役割 任意 共著
    貢献度 任意
    学籍番号 推奨
  5. 岡村 裕彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. 羽地 達次
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Alteration of argyrophilic nucleolar organizer region associated (Ag-NOR) proteins in apoptosis-induced human salivary gland cells and human squamous carcinoma cells.

副題 任意
要約 任意

(英) The level of argyrophilic nucleolar organizer regions (AgNORs) and AgNOR-associated proteins (Ag-NOR proteins) varies with cell activity, including ribosomal biogenesis occurring in proliferating cells. Proteins associated with some AgNORs are detected by a specific silver staining. To investigate a possible relationship between apoptosis and the AgNORs or Ag-NOR proteins, we examined the changes of AgNORs and Ag-NOR proteins during apoptosis in a human salivary gland cell line, HSG cells, and a human oral squamous carcinoma cell line, SCC-25 cells. Apoptosis was induced by treatment of HSG and SCC-25 cells with okadaic acid. Proteins prepared from HSG and SCC-25 cells treated with varying concentrations of okadaic acid (OA) were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by transferring to transfer membranes and staining for Ag-NOR proteins by modified Western blot analysis. Four major bands (110 kDa, 43 kDa, 39kDa, and 37 kDa) were detected in the proteins obtained from the control cells. The level of the 110-kDa protein decreased in the proteins prepared from OA-induced apoptotic cells; however, the reaction intensity of the other three bands was changed in apoptotic cells. An additional band of an 80-kDa Ag-NOR protein appeared and increased in the apoptotic cells. Cellular fractionation of HSG cells and SCC-25 cells was done with or without apoptotic induction. An 80-kDa Ag-NOR protein was detected in the nuclear fraction prepared from the apoptotic cells, while the 110-kDa protein decreased in the nuclear fraction of these cells. The 110-kDa Ag-NOR protein may be nucleolin (C23) as deduced from its AgNOR staining features, including molecular weight. The 80-kDa protein may be the cleavage product of the 110-kDa protein. In the cell-free apoptotic system, in which intact nuclei of HSG cells were incubated with the cytosol fraction of apoptotic HSG and SCC-25 cells, the 80-kDa Ag-NOR protein was detected in nuclei incubated with the cytosol fraction of apoptotic cells, while the level of the 110-kDa protein decreased. The changes of Ag-NOR proteins in nuclei prepared from SCC-25 cells incubated with cytosol fractions prepared from HSG and SCC-25 cells were identical to those of the HSG cells. The alternation of AgNORs in apoptosis-induced HSG cells was also examined using double staining with Hoechst 33342 and silver nitrate. Hoechst staining revealed typical apoptotic nuclei, which exhibited highly fluorescent condensed chromatin in OA-treated HSG cells. Silver grains representing AgNORs were not detected in the cells undergoing apoptosis. The dual-imposition view confirmed that AgNORs, which are visible as dots in nucleoli in the control cells, disappeared from the apoptotic nuclei of HSG cells. Our results indicate that the 110-kDa nucleolar Ag-NOR protein is associated with apoptosis and is cleaved during apoptosis.

キーワード 推奨
  1. (英) Antigens
  2. (英) Antigens, Nuclear
  3. アポトーシス(apoptosis)
  4. (英) Benzimidazoles
  5. (英) Blotting, Western
  6. (英) Carcinoma, Squamous Cell
  7. (英) Cell Line
  8. (英) Cell Nucleus
  9. (英) Chromatin
  10. (英) Coloring Agents
  11. (英) Cytosol
  12. (英) Electrophoresis, Polyacrylamide Gel
  13. (英) Enzyme Inhibitors
  14. (英) Fluorescent Dyes
  15. (英) Humans
  16. 分子量(molecular weight)
  17. (英) Mouth Neoplasms
  18. (英) Nuclear Proteins
  19. (英) Nucleolus Organizer Region
  20. (英) Okadaic Acid
  21. (英) Phosphoproteins
  22. (英) RNA-Binding Proteins
  23. 唾液腺(salivary glands/->キーワード[唾液腺])
  24. (英) Silver
  25. (英) Silver Nitrate
  26. (英) Tumor Cells, Cultured
発行所 推奨
誌名 必須 Journal of Oral Pathology & Medicine(International Association of Oral Pathologists/American Academy of Oral Pathology)
(pISSN: 0904-2512, eISSN: 1600-0714)
ISSN 任意 0904-2512
ISSN: 0904-2512 (pISSN: 0904-2512, eISSN: 1600-0714)
Title: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Title(ISO): J Oral Pathol Med
Supplier: International Association of Oral Pathologists
Publisher: Blackwell
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 30
必須 4
必須 193 199
都市 任意
年月日 必須 2001年 1月 初日
URL 任意
DOI 任意 10.1034/j.1600-0714.2001.300401.x    (→Scopusで検索)
PMID 任意 11302237    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意 2-s2.0-0035094733
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.PublicationTypeList.PublicationType: Journal Article

  2. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't