徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)

1. (英) Nakamura Koji

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2. (英) Shima Hiroshi

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3. (英) Watanabe Masahiko

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4. 羽地 達次

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5. (英) Kikuchi Kunimi

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(英) Molecular cloning and characterization of a novel dual specificity protein phosphatase possibly involved in spermatogenesis.

(英) Dual-specificity protein phosphatases (DSPs) play roles in the regulation of mitogenic signal transduction for extracellular stimulation and the cell cycle. In the present study, we identified a novel DSP, termed TMDP (testis- and skeletal-muscle-specific DSP). Nucleotide sequence analysis of TMDP cDNA indicated that the open reading frame of 597 bp encodes a protein of 198 amino acid residues with a predicted molecular mass of 22.5 kDa. The deduced amino acid sequence contains a motif for a conserved catalytic domain of DSPs and shows highest similarity to human Vaccinia HI-related phosphatase (45.5% identity) but low homology to the mitogen-activated protein kinase phosphatase and CDC25 subfamilies of DSPs. Recombinant TMDP protein exhibited intrinsic phosphatase activity towards both phospho-seryl/threonyl and -tyrosyl residues of myelin basic protein, with similar specific activities in vitro. Northern-blot analysis revealed that TMDP is most abundantly expressed in the testis. The expression in the testis is characterized as follows: (i) TMDP mRNA first appeared 3 weeks after birth, corresponding to the time that meiosis begins; (ii) TMDP mRNA was abundant in fractionated spermatocytes and round spermatids; and (iii) hybridization in situ showed that the TMDP mRNA is localized in spermatocytes and/or spermatids in seminiferous tubules. These data demonstrate that TMDP is a novel DSP abundantly expressed in the testis and suggest that TMDP may be involved in the regulation of meiosis and/or differentiation of testicular germ cells during spermatogenesis.

1. (英) Amino Acid Sequence
2. (英) Animals
3. (英) Base Sequence
4. (英) Cloning, Molecular
5. (英) Dual-Specificity Phosphatases
6. (英) Gene Expression Regulation, Developmental
7. (英) In Situ Hybridization
8. (英) Male
9. (英) Mice
10. (英) Molecular Sequence Data
11. (英) Phosphoprotein Phosphatases
12. (英) Protein Tyrosine Phosphatases
13. (英) RNA, Messenger
14. (英) Recombinant Proteins
15. (英) Spermatocytes
16. (英) Spermatogenesis
17. (英) Substrate Specificity
18. (英) Testis

1. (英) Article.DataBankList.DataBank.DataBankName: GENBANK

2. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB027003

3. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: AB027004

4. (英) Article.PublicationTypeList.PublicationType: Journal Article

5. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

6. (英) OtherID: PMC1220704