徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)

1. 森本 景之

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2. (英) Morimoto Yasuhiro

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3. (英) Ohba Takeshi

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4. (英) Kido Hirofumi

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5. (英) Kobayashi Shigeru / (日) Kobayashi Shigeru

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6. 羽地 達次

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(英) Inhibitors of protein synthesis and RNA synthesis protect against okadaic acid induced apoptosis in human osteosarcoma cell line MG63 cells but not in Saos-2 cells.

(英) In a previous study, we demonstrated that the protein phosphatase inhibitors, okadaic acid and calyculin A, induced apoptosis in human osteosarcoma cell lines, Saos-2 and MG63 cells. In the present study, to determine if new gene transcription and protein synthesis are required for okadaic acid-induced apoptosis in Saos-2 and MG63 cells, the cells were treated for 48h with varying concentrations of the inhibitors of protein or RNA synthesis, i.e., cycloheximide, actinomycin D, and puromycin, in the presence of a fixed dose of okadaic acid. All these reagents in different concentrations prevented the okadaic acid-induced apoptosis in MG63 cells in a dose-dependent fashion. The same concentrations of cycloheximide, actinomycin D, or puromycin alone did not induce any apoptotic features in MG63 cells. However, not all the aforementioned reagents affected okadaic acid-induced apoptosis in Saos-2 cells. Okadaic acid-induced and cycloheximide-prevented apoptosis was shown by phase-contrast microscopy, WST-1 assay, direct visualization of nuclear condensation and fragmentation of chromatin, and the characteristic DNA ladder formation on agarose gel electrophoresis. The present results indicate that the induction of new cell death genes and ongoing protein synthesis may have a role in okadaic acid-induced apoptosis in MG63 cells and that such proteins are not required in Saos-2 cells.

1. アポトーシス(apoptosis)
2. (英) Cell Nucleus
3. (英) Cycloheximide
4. (英) DNA Fragmentation
5. (英) Dactinomycin
6. (英) Enzyme Inhibitors
7. (英) Humans
8. (英) Okadaic Acid
9. (英) Osteosarcoma
10. (英) Protein Synthesis Inhibitors
11. (英) Puromycin
12. RNA(RNA)
13. (英) Tumor Cells, Cultured

1. (英) Article.PublicationTypeList.PublicationType: Journal Article

2. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't