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著作: Murata Takatoshi/Ansai Toshihiro/Takehara Tadamichi/Kobayashi Shigeru/[羽地 達次]/Extracts of Prevotella intermedia and Actinobacillus actinomycetemcomitans inhibit alkaline phosphatase activity in osteoblastic cells in vitro./[Oral Diseases]

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70902
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887060
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2018年2月6日(火) 14:25:25
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三木 ちひろ
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[副研究部長]/[徳島大学.大学院医歯薬学研究部]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨 研究
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)
著者 必須
  1. (英) Murata Takatoshi
    役割 任意 共著
    貢献度 任意
    学籍番号 推奨
  2. (英) Ansai Toshihiro
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Takehara Tadamichi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Kobayashi Shigeru
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 羽地 達次
    役割 任意 共著
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Extracts of Prevotella intermedia and Actinobacillus actinomycetemcomitans inhibit alkaline phosphatase activity in osteoblastic cells in vitro.

副題 任意
要約 任意

(英) In order to study the effects of sonicated extracts from Prevotella intermedia, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and other oral-related bacteria, as well as Escherichia coli on bone formation, clone MC3T3-E1 cells, which have retained osteoblastic activity, were cultured with various bacterial extracts. The addition of the sonicated extracts from Prevotella intermedia and Actinobacillus actinomycetemcomitans decreased the alkaline phosphatase activity in a dose-dependent fashion over the concentration range of 1-1000 ng ml-1 compared with the control. By contrast, the sonicated extracts from other oral bacteria including Porphyromonas gingivalis, Capnocytophaga ochracea, Streptococcus milleri and Streptococcus sanguis, and Escherichia coli did not decrease the alkaline phosphatase activity even in the presence of 100 ng ml-1 protein. The addition of Prevotella intermedia and Actinobacillus actinomycetemcomitans extracts that had been treated with heat and trypsin to the cell cultures also inhibited alkaline phosphatase activity in the cells, suggesting that inhibitory factors are not proteinaceous. Polymyxin B did not change the alkaline phosphatase activity in the cells treated with the extracts from Prevotella intermedia and Actinobacillus actinomycetemcomitans, suggesting that the inhibitory activity of the extracts is not lipopolysaccharide. The inhibitory effect of both extracts was observed in the molecular mass over 290 kDa eluted from Sephadex G-200 column. The inhibitory substances of Prevotella intermedia were partially purified and showed broad band with estimated molecular weight of 170-190 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results indicate that Prevotella intermedia and Actinobacillus actinomycetemcomitans may play an important role in inhibiting bone formation as well as in stimulating bone resorption.

キーワード 推奨
  1. (英) 3T3 Cells
  2. (英) Aggregatibacter actinomycetemcomitans
  3. (英) Alkaline Phosphatase
  4. (英) Animals
  5. (英) Bacterial Proteins
  6. (英) Clone Cells
  7. (英) Culture Media, Conditioned
  8. (英) Dose-Response Relationship, Drug
  9. (英) Enzyme Inhibitors
  10. (英) Lipopolysaccharides
  11. (英) Mice
  12. 分子量(molecular weight)
  13. (英) Osteoblasts
  14. (英) Osteogenesis
  15. (英) Prevotella intermedia
  16. (英) Sonication
発行所 推奨
誌名 必須 Oral Diseases(Munksgaard)
(pISSN: 1354-523X, eISSN: 1601-0825)
ISSN 任意 1354-523X
ISSN: 1354-523X (pISSN: 1354-523X, eISSN: 1601-0825)
Title: Oral diseases
Title(ISO): Oral Dis
Publisher: Blackwell
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 3
必須 2
必須 106 112
都市 任意
年月日 必須 1997年 1月 初日
URL 任意
DOI 任意 10.1111/j.1601-0825.1997.tb00021.x    (→Scopusで検索)
PMID 任意 9467351    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.PublicationTypeList.PublicationType: Comparative Study

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't