徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)
2. 徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座(2007年4月1日〜)

1. (英) Kito Shinji

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2. (英) Shimizu Katsuhide

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3. 岡村 裕彦

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4. 吉田 賀弥([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健支援学]/[徳島大学.歯学部.口腔保健学科.口腔保健支援学講座])

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5. (英) Fujita Michi

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6. 森本 景之

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7. (英) Morimoto Yasuhiro

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8. (英) Ohba Takeshi

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9. 羽地 達次

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(英) Cleavage of nucleolin and argyrophilic nucleolar organizer region associated proteins in

(英) To investigate the behavior of nuclear proteins in apoptotic cells, we examined the changes in nucleolin and proteins of the nucleolar organizing region during apoptosis in human osteoblastic cell lines, Saos-2 and MG63. Apoptosis was induced by treatment of these cells with okadaic acid. Proteins prepared from apoptotic cells were subjected to Western blot analysis and a modified Western blot method using silver nitrate. The anti-nucleolin antibody recognized the 110-kDa band and the staining intensity of this band decreased in the proteins prepared from the okadaic acid-treated apoptotic cells. The additional band of an 80-kDa was also detected in the proteins prepared from the apoptotic cells. Two major silver nitrate-stained bands, 110-kDa and 37-kDa, were detected among the proteins obtained from control cells. Like the Western blot analysis, the intensity of the 110-kDa silver nitrate-staining band decreased; an 80-kDa band appeared and its staining intensity increased in the lysate from the okadaic acid-treated cells. The signal intensity of the 37-kDa protein did not change in the sample from the apoptotic cells. In a cell-free apoptotic system, the 80-kDa protein was also detected and the amount of the 110-kDa protein decreased in the extract of Saos-2 cell nuclei incubated with apoptotic cytosol. The change in nucleolin in Saos-2 cells induced to undergo apoptosis was examined by an immunocytochemical procedure using the anti-nucleolin antibody and Hoechst 33342. Nucleolin was visible as dots in nucleoli in the control cells; however, it was not detected in the cells undergoing apoptosis. The dual-exposure view of Hoechst 33342 and anti-nucleolin staining cells confirmed that nucleolin had disappeared from the apoptotic nuclei of Saos-2.

1. (英) Apoptosis
2. (英) Blotting, Western
3. (英) Cell Fractionation
4. (英) Cell Line
5. (英) Cell Nucleolus
6. (英) Cell Nucleus
7. (英) Cell-Free System
8. (英) DNA Fragmentation
9. (英) Enzyme Inhibitors
10. (英) Fluorescent Dyes
11. (英) Humans
12. (英) Immunohistochemistry
13. (英) Nuclear Proteins
14. (英) Nucleolus Organizer Region
15. (英) Okadaic Acid
16. (英) Osteoblasts
17. (英) Phosphoproteins
18. (英) RNA-Binding Proteins
19. (英) Silver Nitrate

1. (英) Article.Affiliation: Department of Histology and Oral Histology, Kyushu Dental College, Kitakyushu, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't