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著作: [藤原 奈津美]/Yamashita Shohei/Okamoto Motoki/Cooley Marion A/[尾崎 和美]/Everett Eric T/Suzuki Maiko/Perfluorooctanoic acid-induced cell death via the dual roles of ROS-MAPK/ERK signaling in ameloblast-lineage cells./[Ecotoxicology and Environmental Safety]

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EID
401510
EOID
1086150
Map
0
LastModified
2023年8月22日(火) 19:15:18
Operator
大家 隆弘
Avail
TRUE
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0
Owner
藤原 奈津美
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 藤原 奈津美([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健医療管理学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Yamashita Shohei
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Okamoto Motoki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Cooley Marion A
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 尾崎 和美([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健支援学]/[徳島大学.歯学部.口腔保健学科.口腔保健支援学講座])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Everett Eric T
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Suzuki Maiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Perfluorooctanoic acid-induced cell death via the dual roles of ROS-MAPK/ERK signaling in ameloblast-lineage cells.

副題 任意
要約 任意

(英) Perfluorooctanoic acid (PFOA) is an artificial fluorinated organic compound that has generated increased public attention due to its potential health hazards. Unsafe levels of PFOA exposure can affect reproduction, growth and development. During tooth enamel development (amelogenesis), environmental factors including fluoride can cause enamel hypoplasia. However, the effects of PFOA on ameloblasts and tooth enamel formation remain largely unknown. In the present study we demonstrate several PFOA-mediated cell death pathways (necrosis/necroptosis, and apoptosis) and assess the roles of ROS-MAPK/ERK signaling in PFOA-mediated cell death in mouse ameloblast-lineage cells (ALC). ALC cells were treated with PFOA. Cell proliferation and viability were analyzed by MTT assays and colony formation assays, respectively. PFOA suppressed cell proliferation and viability in a dose dependent manner. PFOA induced both necrosis (PI-positive cells) and apoptosis (cleaved-caspase-3, γH2AX and TUNEL-positive cells). PFOA significantly increased ROS production and up-regulated phosphor-(p)-ERK. Addition of ROS inhibitor N-acetyl cysteine (NAC) suppressed p-ERK and decreased necrosis, and increased cell viability compared to PFOA alone, whereas NAC did not change apoptosis. This suggests that PFOA-mediated necrosis was induced by ROS-MAPK/ERK signaling, but apoptosis was not associated with ROS. Addition of MAPK/ERK inhibitor PD98059 suppressed necrosis and increased cell viability compared to PFOA alone. Intriguingly, PD98059 augmented PFOA-mediated apoptosis. This suggests that p-ERK promoted necrosis but suppressed apoptosis. Addition of the necroptosis inhibitor Necrostatin-1 restored cell viability compared to PFOA alone, while pan-caspase inhibitor Z-VAD did not mitigate PFOA-mediated cell death. These results suggest that 1) PFOA-mediated cell death was mainly caused by necrosis/necroptosis by ROS-MAPK/ERK signaling rather than apoptosis, 2) MAPK/ERK signaling plays the dual roles (promoting necrosis and suppressing apoptosis) under PFOA treatment. This is the initial report to indicate that PFOA could be considered as a possible causative factor for cryptogenic enamel malformation. Further studies are required to elucidate the mechanisms of PFOA-mediated adverse effects on amelogenesis.

キーワード 推奨
  1. (英) Mice
  2. (英) Animals
  3. (英) Reactive Oxygen Species
  4. (英) Ameloblasts
  5. (英) Cell Death
  6. (英) Necrosis
発行所 推奨
誌名 必須 Ecotoxicology and Environmental Safety(International Academy of Environmental Safety/International Society of Ecotoxicology and Environmental Safety)
(pISSN: 0147-6513, eISSN: 1090-2414)
ISSN 任意 1090-2414
ISSN: 0147-6513 (pISSN: 0147-6513, eISSN: 1090-2414)
Title: Ecotoxicology and environmental safety
Title(ISO): Ecotoxicol Environ Saf
Publisher: Academic Press
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 260
必須
必須
都市 任意
年月日 必須 2023年 6月 2日
URL 任意
DOI 任意 10.1016/j.ecoenv.2023.115089    (→Scopusで検索)
PMID 任意 37271104    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) PublicationType: Journal Article