著作: 福富 茉由/上殿 千晴/藤井 亜紀/[佐藤 陽一]/Lrriq1 is an essential factor for fertility by suppressing apoptosis./[Journal of Assisted Reproduction and Genetics]/[佐藤 陽一]
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種別 | 必須 | 学術論文(審査論文) | ||||||||||||||||||||||||||||||||||||
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言語 | 必須 | 英語 | ||||||||||||||||||||||||||||||||||||
招待 | 推奨 | |||||||||||||||||||||||||||||||||||||
審査 | 推奨 | Peer Review | ||||||||||||||||||||||||||||||||||||
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共著種別 | 推奨 | |||||||||||||||||||||||||||||||||||||
学究種別 | 推奨 | |||||||||||||||||||||||||||||||||||||
組織 | 推奨 | |||||||||||||||||||||||||||||||||||||
著者 | 必須 |
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題名 | 必須 |
(英) Lrriq1 is an essential factor for fertility by suppressing apoptosis. |
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副題 | 任意 | |||||||||||||||||||||||||||||||||||||
要約 | 任意 |
(英) Leucine-rich repeats and IQ motif containing 1 (LRRIQ1) gene is reportedly associated with plasma inhibin B levels. However, the function of LRRIQ1 remains unknown. In this study, we generated Lrriq1 knockout mice (Lrriq1 mice) and examined the effects of LRRIQ1 on inhibin B and fertility. Lrriq1 mice were generated using CRISPR/Cas9 genome editing technology. The expression of Inhibin B was examined by Western blotting using a protein extracted from the testis of a 3-month-old male mouse. Mating experiments were conducted using 7-week-old Lrriq1 mice and wild-type (WT) mice to examine fertility. Sperm concentration and sperm motility were measured using 3-month-old male mice. Expression analysis of inhibin B revealed that Lrriq1 mice exhibited reduced mRNA and protein levels of inhibin alpha (Inha), which constitutes the α subunit. In the mating experiment, the litter size of Lrriq1 male mice was 4.3 ± 2.9, which was significantly lower than that of WT male mice (8.3 ± 1.3) (p < 0.001). No difference in sperm count was observed between Lrriq1 and WT male mice; however, sperm motility (%) was significantly reduced in Lrriq1 mice (48.4 ± 4.9) when compared with WT mice (70.2 ± 4.7) (p < 0.001). Based on TUNEL staining, the testes and epididymal sperm of Lrriq1 mice showed high numbers of apoptosis-positive cells. Lrriq1 knockout reduced sperm motility and litter size by inducing apoptosis of testicular germ cells and epididymal sperm. |
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キーワード | 推奨 |
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誌名 | 必須 |
Journal of Assisted Reproduction and Genetics(International Working Group on Preimplantation Genetics)
(pISSN: 1058-0468, eISSN: 1573-7330)
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巻 | 必須 | 39 | ||||||||||||||||||||||||||||||||||||
号 | 必須 | 11 | ||||||||||||||||||||||||||||||||||||
頁 | 必須 | 2647 2657 | ||||||||||||||||||||||||||||||||||||
都市 | 任意 | |||||||||||||||||||||||||||||||||||||
年月日 | 必須 | 2022年 11月 初日 | ||||||||||||||||||||||||||||||||||||
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DOI | 任意 | 10.1007/s10815-022-02623-9 (→Scopusで検索) | ||||||||||||||||||||||||||||||||||||
PMID | 任意 | 36129630 (→Scopusで検索) | ||||||||||||||||||||||||||||||||||||
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Scopus | 任意 | |||||||||||||||||||||||||||||||||||||
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指導教員 | 推奨 |
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備考 | 任意 |
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