徳島大学 教育・研究者情報データベース(EDB)

1. (英) Shono Kenji

   役割	任意
   貢献度	任意
   学籍番号	推奨

2. 溝渕 佳史

   役割	任意
   貢献度	任意
   学籍番号	推奨

3. 山口 泉([徳島大学.病院.診療科.脳·神経·精神科.脳神経外科])

   役割	任意
   貢献度	任意
   学籍番号	推奨

4. 中島 公平([徳島大学.病院.先端医科医療開発研究プロジェクト]/[徳島大学.病院.診療科.脳·神経·精神科.脳神経外科])

   役割	任意
   貢献度	任意
   学籍番号	推奨

5. (英) Fujiwara Yuri

   役割	任意
   貢献度	任意
   学籍番号	推奨

6. 藤原 敏孝([徳島大学.病院.中央診療施設等.てんかんセンター])

   役割	任意
   貢献度	任意
   学籍番号	推奨

7. (英) Kitazato Keiko

   役割	任意
   貢献度	任意
   学籍番号	推奨

8. 松﨑 和仁

   役割	任意
   貢献度	任意
   学籍番号	推奨

9. 宇都 義浩([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命コース])

   役割	任意
   貢献度	任意
   学籍番号	推奨

10. (英) Sampetrean Oltea

    役割	任意
    貢献度	任意
    学籍番号	推奨

11. (英) Saya Hideyuki

    役割	任意
    貢献度	任意
    学籍番号	推奨

12. 髙木 康志([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.脳神経外科学])

    役割	任意
    貢献度	任意
    学籍番号	推奨

(英) Elevated cellular PpIX potentiates sonodynamic therapy in a mouse glioma stem cell-bearing glioma model by downregulating the Akt/NF-κB/MDR1 pathway.

(英) Glioblastoma (GBM) has high mortality rates because of extreme therapeutic resistance. During surgical resection for GBM, 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PpIX) fluorescence is conventionally applied to distinguish GBM. However, surgical intervention is insufficient for high invasive GBM. Sonodynamic therapy (SDT) combined with low-intensity ultrasonication (US) and PpIX, as a sonosensitizer, is an emerging and promising approach, although its efficacy is limited. Based on our previous study that down-regulation of multidrug resistant protein (MDR1) in GBM augmented the anti-tumor effects of chemotherapy, we hypothesized that elevation of cellular PpIX levels by down-regulation of MDR1 enhances anti-tumor effects by SDT. In high invasive progeny cells from mouse glioma stem cells (GSCs) and a GSC-bearing mouse glioma model, we assessed the anti-tumor effects of SDT with a COX-2 inhibitor, celecoxib. Down-regulation of MDR1 by celecoxib increased cellular PpIX levels, as well as valspodar, an MDR1 inhibitor, and augmented anti-tumor effects of SDT. MDR1 down-regulation via the Akt/NF-κB pathway by celecoxib was confirmed, using an NF-κB inhibitor, CAPÉ. Thus, elevation of cellar PpIX by down-regulation of MDR1 via the Akt/NF-κB pathway may be crucial to potentiate the efficacy of SDT in a site-directed manner and provide a promising new therapeutic strategy for GBM.

1. (英) ATP Binding Cassette Transporter, Subfamily B, Member 1
2. (英) Aminolevulinic Acid
3. (英) Animals
4. (英) Cell Line, Tumor
5. (英) Down-Regulation
6. (英) Glioblastoma
7. (英) Glioma
8. (英) Male
9. (英) Mice
10. (英) Mice, Inbred C57BL
11. (英) NF-kappa B
12. (英) Neoplastic Stem Cells
13. (英) Photosensitizing Agents
14. (英) Proto-Oncogene Proteins c-akt
15. (英) Protoporphyrins
16. (英) Signal Transduction
17. (英) Ultrasonic Therapy

1. (英) PublicationType: Journal Article

2. (英) PublicationType: Research Support, Non-U.S. Gov't