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著作: Tsukahara Narutoshi/[村上 明一]/Motohashi Maiko/Nakayama Hiroshi/Kondo Yoshiro/Ito Yuji/Azuma Takachika/Kishimoto Hidehiro/An alpaca single-domain antibody (VHH) phage display library constructed by CDR shuffling provided high-affinity VHHs against desired protein antigens./[International Immunology]

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EID
388406
EOID
1051269
Map
0
LastModified
2022年8月8日(月) 13:40:00
Operator
村上 明一
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村上 明一
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Tsukahara Narutoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 村上 明一([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Motohashi Maiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Nakayama Hiroshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Kondo Yoshiro
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Ito Yuji
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    貢献度 任意
    学籍番号 推奨
  7. (英) Azuma Takachika
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. (英) Kishimoto Hidehiro
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) An alpaca single-domain antibody (VHH) phage display library constructed by CDR shuffling provided high-affinity VHHs against desired protein antigens.

副題 任意
要約 任意

(英) Antigen-combining sites of the camelid heavy-chain antibody variable domain (VHH) are constructed by three complementarity-determining regions (CDR1, CDR2 and CDR3). We prepared cDNA using mRNA extracted from peripheral lymphocytes of alpacas that had been non-immunized or immunized with human serum albumin (HSA). The VHH gene fragments encoding the amino-terminal half-containing CDR1 as well as CDR2 and the carboxy-terminal half-containing CDR3 were amplified independently by PCR, and then full-length VHH gene fragments were generated by overlap extension PCR and cloned into the phagemid vector. This protocol, referred to as CDR shuffling, allowed us to construct an alpaca VHH phage display library possessing repertoires different from those naturally occurring in animals. We asked, first, whether this library was able to provide the functional VHH fragments against HSA, an immunized antigen, and obtained 29 anti-HSA VHH clones, 41% possessed KD values of lower than 10-8 M, 5 of which had KD values of 10-10 M. We also obtained VHH clones against non-immunized protein antigens such as cardiac troponin T and I, Ebola virus glycoprotein 1 and human immunoglobulin G by biopanning. We compared the amino acid sequences and affinities and found that 43% of VHHs had KD values of less than 10-8 M, although those having KD values of 10-10 M were unavailable. These results suggested that the CDR-shuffled VHH phage display library could potentially provide VHHs against non-immunized protein antigens with similar levels of affinities to those against immunized antigens.

キーワード 推奨
  1. (英) Animals
  2. (英) Antigens
  3. (英) Bacteriophages
  4. (英) Camelids, New World
  5. (英) Gene Library
  6. (英) Humans
  7. (英) Immunoglobulin Heavy Chains
  8. (英) Single-Domain Antibodies
発行所 推奨
誌名 必須 International Immunology([Oxford University Press])
(pISSN: 0953-8178, eISSN: 1460-2377)
ISSN 任意 1460-2377
ISSN: 0953-8178 (pISSN: 0953-8178, eISSN: 1460-2377)
Title: International immunology
Title(ISO): Int Immunol
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (医中誌Web  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 34
必須 8
必須 421 434
都市 任意
年月日 必須 2022年 7月 26日
URL 任意
DOI 任意 10.1093/intimm/dxac022    (→Scopusで検索)
PMID 任意 35689594    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) PublicationType: Journal Article