徳島大学 教育・研究者情報データベース(EDB)

1. (英) Namula Zhao

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2. (英) Wittayarat Manita

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3. (英) Do Lanh Thi Kim / (日) DO THI KIM LANH / (読) ど てぃ きむ らん

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4. (英) Van Nguyen Thanh

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5. (英) Lin Qingyi / (日) 林 青怡 / (読) りん せいい

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6. (英) Takebayashi Koki

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7. 平田 真樹([徳島大学.バイオイノベーション研究所])

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8. 谷原 史倫([徳島大学.バイオイノベーション研究所])

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9. 音井 威重([徳島大学.バイオイノベーション研究所])

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(英) Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system

(英) , and

(日) were simultaneously introduced into the oocytes that had been incubated for 40, 42, and 44 h from the start of the IVM culture. Electroporation with three gRNAs at 40 h and 42 h during IVM culture decreased the blastocyst formation rates and did not improve the mutation rates and target number of biallelic mutations in the resulting blastocysts. The blastocyst formation rate, mutation rates, and target numbers in the resulting blastocysts from oocytes treated by electroporation at 44 h of IVM culture were similar to those of control zygotes electroporated at 13 h after the initiation of IVF. In conclusion, multiple gene editing efficiency in the resulting blastocysts was comparable between oocytes electroporated before and after the fertilization, indicating that oocytes with completed maturation time may allow better functioning of materials accepting gene editing application.

1. (英) PublicationType: Journal Article