徳島大学 教育・研究者情報データベース(EDB)

1. (英) Tentaku Aya / (日) 天宅あや / (読) てんたくあや

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2. 栗栖 修作([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.細胞生物学])

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3. (英) Sejima Kurumi / (日) 瀬嶋来実 / (読) せじまくるみ

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4. (英) Nagao Toshiki / (日) 長尾俊紀 / (読) ながおとしき

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5. 髙橋 章([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.予防環境栄養学])

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6. 米村 重信([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.細胞生物学])

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(英) Proximal deposition of collagen IV by fibroblasts contributes to basement membrane formation by colon epithelial cells invitro.

(英) The basement membrane (BM) underlying epithelial tissue is a thin layer of extracellular matrix that governs tissue integrity and function. Epithelial BMs are generally assembled using BM components secreted from two origins: epithelium and stroma. Although denovo BM formation involves self-assembly processes of large proteins, it remains unclear how stroma-derived macromolecules are transported and assembled, specifically in the BM region. In this study, we established an invitro co-culture model of BM formation in which DLD-1 human colon epithelial cells were cultured on top of collagen I gel containing human embryonic OUMS-36T-2 fibroblasts as stromal cells. A distinct feature of our system is represented by OUMS-36T-2 cells which are almost exclusively responsible for synthesis of collagen IV, a major BM component. Exploiting this advantage, we found that collagen IV incorporation was significantly impaired in culture conditions where OUMS-36T-2 cells were not allowed to directly contact DLD-1 cells. Soluble collagen IV, once diluted in the culture medium, did not accumulate in the BM region efficiently. Live imaging of fluorescently tagged collagen IV revealed that OUMS-36T-2 cells deposited collagen IV aggregates directly onto the basal surface of DLD-1 cells. Collectively, these results indicate a novel mode of collagen IV deposition in which fibroblasts proximal to epithelial cells exclusively contribute to collagen IV assembly during BM formation.

1. コラーゲン(collagen)
2. 細胞外マトリックス(extracellular matrix)
3. (英) co-culture / (日) 共培養
4. (英) colon epithelium / (日) 腸管上皮
5. (英) fibroblast / (日) 線維芽細胞

1. (英) PublicationType: Journal Article