徳島大学 教育・研究者情報データベース(EDB)

1. 髙須 千絵([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学])

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2. 宮崎 克己([徳島大学.病院.診療科.外科.消化器・移植外科])

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3. 吉川 幸造

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4. 西 正暁([徳島大学.大学院医歯薬学研究部.医学域.連携研究部門(医学域).寄附講座系(医学域).実践地域診療・医科学])

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5. 徳永 卓哉([徳島大学.病院.中央診療施設等.地域外科診療部])

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6. 柏原 秀也([徳島大学.病院.先端医科医療開発研究プロジェクト]/[徳島大学.病院.診療科.外科.消化器・移植外科])

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7. 良元 俊昭([徳島大学.病院.栄養部])

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8. 小川 博久([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.疾患病理学])

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9. 森根 裕二([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学]/[徳島大学.病院.診療科.外科.消化器・移植外科])

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10. 島田 光生([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学])

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(英) Effect of TU-100 on Peyer's patches in a bacterial translocation rat model.

(英) Daikenchuto (TU-100), a Japanese herbal medicine, is widely used for various gastrointestinal diseases. We have previously reported that TU-100 suppresses CPT-11-induced bacterial translocation (BT) by maintaining the diversity of the microbiome. In this study we show that TU-100 modulates the immune response during BT by inducing PD-1 expression in Peyer's patches. Eighteen male Wistar rats were divided into four groups: a control group; a control + TU-100 group, given TU-100 1000 mg/kg orally for 5 d; a BT group, given CPT-11 250 mg/kg intra-peritoneal for 2 d; and a TU-100 group, given TU-100 1000 mg/kg orally for 5 d with CPT-11 250 mg/kg intra-peritoneal on days 4 and 5. The size of Peyer's patch was significantly bigger in the BT group compared to the control group (9.0 104 m vs 29.4 104 m, < .05), but improved in the TU-100 group (15.4 104 m, < .005). TU-100 significantly induced PD-1 expression in Peyer's patch compared to the control group and the BT group (control vs BT vs TU-100 = 4.3 4.9 vs 5.1 10.3 vs 17.9 17.8). The CD4 cells were increased in the BT group ( < .05) compared to the control group but decreased in the TU-100 group. The Foxp3 cells were increased in the BT group compared to the control group ( < .05), and further increased in the TU-100 group compared to the BT group. CPT-11 significantly increased TLR4, NF- , TNF- mRNA expressions in the BT group. TU-100 cotreatment significantly reversed these mRNA expressions. TU-100 may have a protective effect against BT through PD-1 expression in Peyer's patch.

1. (英) Article.ELocationID: 10.1002/ags3.12460

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) KeywordList.Keyword: Kampo

4. (英) KeywordList.Keyword: herbal medicine

5. (英) KeywordList.Keyword: inflammatory cytokine

6. (英) CoiStatement: Funding: This study was partly supported by a Cancer Research Project Cooperated by TAIHO Pharmaceutical Co., Ltd. and the University of Tokushima and by Japan Society for the promotion of Science (Grantnid for Scientific Research B: No. 23791536). Mitsuo Shimada received a research grant from Taiho Pharmaceutical Co. Ltd.C.T. and K.Y. designed the experiment. C.T., H.K and T.Y performed the experiments and analyzed the data. C.T wrote the manuscript in discussions with M.N and T.T. H.O analyzed IHC experiment with C.T.. K.M performed the revised experiments with C.T.. S.M. supervised the whole project and Y.M. directed the revised project.