徳島大学 教育・研究者情報データベース(EDB)

1. (英) Gao Luping / (日) 高 露萍 / (読) こう ろへい

   役割	任意
   貢献度	任意
   学籍番号	推奨	****

2. 森根 裕二([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学]/[徳島大学.病院.診療科.外科.消化器・移植外科])

   役割	任意
   貢献度	任意
   学籍番号	推奨

3. 山田 眞一郎([徳島大学.病院.診療科.外科.消化器・移植外科])

   役割	任意
   貢献度	任意
   学籍番号	推奨

4. 齋藤 裕([徳島大学.病院.診療科.外科.消化器・移植外科])

   役割	任意
   貢献度	任意
   学籍番号	推奨

5. 池本 哲也([徳島大学.病院.中央診療施設等.安全管理部]/[徳島大学.病院.診療科.外科.消化器・移植外科])

   役割	任意
   貢献度	任意
   学籍番号	推奨

6. 徳田 和憲([徳島大学.病院.診療科.第一外科])

   役割	任意
   貢献度	任意
   学籍番号	推奨

7. 髙須 千絵([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

8. 宮崎 克己([徳島大学.病院.診療科.外科.消化器・移植外科])

   役割	任意
   貢献度	任意
   学籍番号	推奨

9. 島田 光生([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

(英) Nrf2 signaling promotes cancer stemness, migration, and expression of ABC transporter genes in sorafenib-resistant hepatocellular carcinoma cells.

(英) As a multiple tyrosine kinase inhibitor, sorafenib is widely used to treat hepatocellular carcinoma (HCC), but patients frequently face resistance problems. Because the mechanism controlling sorafenib-resistance is not well understood, this study focused on the connection between tumor characteristics and the Nrf2 signaling pathway in a sorafenib-resistant HCC cell line. A sorafenib-resistant HCC cell line (Huh7) was developed by increasing the dose of sorafenib in the culture medium until the target concentration was reached. Cell morphology, migration/invasion rates, and expression of stemness-related and ATP-binding cassette (ABC) transporter genes were compared between sorafenib-resistant Huh7 cells and parental Huh7 cells. Next, a small interfering RNA was used to knock down Nrf2 expression in sorafenib-resistant Huh7 cells, after which cell viability, stemness, migration, and ABC transporter gene expression were examined again. Proliferation, migration, and invasion rates of sorafenib-resistant Huh7 cells were significantly increased relative to the parental cells with or without sorafenib added to the medium. The expression levels of stemness markers and ABC transporter genes were up-regulated in sorafenib-resistant cells. After Nrf2 was knocked down in sorafenib-resistant cells, cell migration and invasion rates were reduced, and expression levels of stemness markers and ABC transporter genes were reduced. Nrf2 signaling promotes cancer stemness, migration, and expression of ABC transporter genes in sorafenib-resistant HCC cells.

1. (英) ATP-Binding Cassette Transporters
2. (英) Antineoplastic Agents
3. (英) Carcinoma, Hepatocellular
4. (英) Cell Line, Tumor
5. (英) Cell Movement
6. (英) Cell Proliferation
7. (英) Cell Survival
8. (英) Drug Resistance, Neoplasm
9. (英) Gene Expression
10. (英) Gene Expression Regulation, Neoplastic
11. (英) Gene Knockdown Techniques
12. (英) Humans
13. (英) Liver Neoplasms
14. (英) NF-E2-Related Factor 2
15. (英) Protein Kinase Inhibitors
16. (英) Signal Transduction
17. (英) Sorafenib
18. (英) Transfection

1. (英) Article.ELocationID: 10.1371/journal.pone.0256755

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

4. (英) CoiStatement: We state the potential conflicts of interest with regard to our study as follows: Mitsuo Shimada declares receiving unrestricted research grant from Bayer Yakuhin, CO. LTD., Japan. All other authors report no conflict of interest. This does not alter our adherence to PLOS ONE policies on sharing data and materials.