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著作: Penrose M. Harrison/Katsurada Akemi/Miyata Kayoko/[漆原 真樹]/Satou Ryousuke/STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells./[Journal of the Renin-Angiotensin-Aldosterone System : JRAAS]

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EID
380007
EOID
1015390
Map
0
LastModified
2021年8月20日(金) 13:40:32
Operator
三木 ちひろ
Avail
TRUE
Censor
0
Owner
漆原 真樹
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Penrose M. Harrison
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Katsurada Akemi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Miyata Kayoko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 漆原 真樹([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.小児科学]/[徳島大学.病院.診療科.小児·周産·女性科.小児科])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Satou Ryousuke
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells.

副題 任意
要約 任意

(英) Intrarenal interferon-γ significantly contributes to the development of glomerular injury in which angiotensinogen and monocyte chemoattractant protein 1 levels are elevated. However, the exact nature of the role that interferon-γ plays in regulating angiotensinogen and monocyte chemoattractant protein 1 expression has not been fully delineated. Therefore, the aim of this study was to investigate the role that interferon-γ plays in angiotensinogen and monocyte chemoattractant protein 1 expression. Primary cultured rat mesangial cells were treated with 0-20 ng/mL interferon-γ for 2, 8 or 24 hours. Expression levels of angiotensinogen, monocyte chemoattractant protein 1, suppressors of cytokine signaling 1, an intracellular suppressor of Janus kinase-signal transducers and activators of transcription signaling and activity of the Janus kinase-signal transducers and activators of transcription pathway were evaluated by reverse transcriptase polymerase chain reaction and western blot analysis. Interferon-γ increased angiotensinogen expression in mesangial cells with maximal augmentation observed following 5 ng/mL interferon-γ at 8 hours of treatment (1.87 ± 0.05, mRNA, relative ratio). Further increases were reduced or absent using higher concentrations of interferon-γ. Following treatments, monocyte chemoattractant protein 1 expression was induced in a linear dose-dependent manner (6.85 ± 0.62-fold by 20 ng/mL interferon-γ at 24 hours). In addition, interferon-γ induced STAT1 phosphorylation and suppressors of cytokine signaling 1 expression in a linear dose-dependent manner. The suppression of STAT1 and suppressors of cytokine signaling 1 expression by small interference RNAs facilitated an increase in interferon-γ-induced angiotensinogen expression, indicating that these two factors negatively regulate angiotensinogen expression. In contrast, the increase in interferon-γ-induced monocyte chemoattractant protein 1 expression was attenuated in STAT1-deficient mesangial cells, suggesting that STAT1 positively regulates monocyte chemoattractant protein 1 expression in mesangial cells. These results demonstrate that while interferon-γ increases both angiotensinogen and monocyte chemoattractant protein 1 expression, STAT1 plays an opposing role in the regulation of each factor in mesangial cells.

キーワード 推奨
  1. (英) Angiotensinogen
  2. (英) Animals
  3. (英) Cells, Cultured
  4. (英) Chemokine CCL2
  5. (英) Interferon-gamma
  6. 男性(male)
  7. (英) Mesangial Cells
  8. (英) Models, Biological
  9. (英) Rats, Sprague-Dawley
  10. (英) STAT1 Transcription Factor
  11. (英) STAT3 Transcription Factor
  12. (英) Suppressor of Cytokine Signaling 1 Protein
発行所 推奨
誌名 必須 Journal of the Renin-Angiotensin-Aldosterone System : JRAAS(Sage Publication)
(pISSN: 1470-3203, eISSN: 1752-8976)
ISSN 任意 1752-8976
ISSN: 1470-3203 (pISSN: 1470-3203, eISSN: 1752-8976)
Title: Journal of the renin-angiotensin-aldosterone system : JRAAS
Title(ISO): J Renin Angiotensin Aldosterone Syst
Publisher: SAGE Publications
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 21
必須 3
必須 1470320320946527 1470320320946527
都市 任意
年月日 必須 2020年 8月 3日
URL 任意
DOI 任意 10.1177/1470320320946527    (→Scopusで検索)
PMID 任意 32741247    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意 2-s2.0-85088906311
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1177/1470320320946527

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) MedlineDate: 2020 Jul-Sep

  4. (英) KeywordList.Keyword: Interferon-γ

  5. (英) KeywordList.Keyword: JAK-STAT pathway

  6. (英) KeywordList.Keyword: MCP-1

  7. (英) KeywordList.Keyword: angiotensinogen

  8. (英) KeywordList.Keyword: mesangial cell