著作: Penrose M. Harrison/Katsurada Akemi/Miyata Kayoko/[漆原 真樹]/Satou Ryousuke/STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells./[Journal of the Renin-Angiotensin-Aldosterone System : JRAAS]
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種別 | 必須 | 学術論文(審査論文) | |||||||||||||||||||||||||||||||||||||||||||||
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言語 | 必須 | 英語 | |||||||||||||||||||||||||||||||||||||||||||||
招待 | 推奨 | ||||||||||||||||||||||||||||||||||||||||||||||
審査 | 推奨 | ||||||||||||||||||||||||||||||||||||||||||||||
カテゴリ | 推奨 | ||||||||||||||||||||||||||||||||||||||||||||||
共著種別 | 推奨 | ||||||||||||||||||||||||||||||||||||||||||||||
学究種別 | 推奨 | ||||||||||||||||||||||||||||||||||||||||||||||
組織 | 推奨 | ||||||||||||||||||||||||||||||||||||||||||||||
著者 | 必須 |
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題名 | 必須 |
(英) STAT1 regulates interferon-γ-induced angiotensinogen and MCP-1 expression in a bidirectional manner in primary cultured mesangial cells. |
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副題 | 任意 | ||||||||||||||||||||||||||||||||||||||||||||||
要約 | 任意 |
(英) Intrarenal interferon-γ significantly contributes to the development of glomerular injury in which angiotensinogen and monocyte chemoattractant protein 1 levels are elevated. However, the exact nature of the role that interferon-γ plays in regulating angiotensinogen and monocyte chemoattractant protein 1 expression has not been fully delineated. Therefore, the aim of this study was to investigate the role that interferon-γ plays in angiotensinogen and monocyte chemoattractant protein 1 expression. Primary cultured rat mesangial cells were treated with 0-20 ng/mL interferon-γ for 2, 8 or 24 hours. Expression levels of angiotensinogen, monocyte chemoattractant protein 1, suppressors of cytokine signaling 1, an intracellular suppressor of Janus kinase-signal transducers and activators of transcription signaling and activity of the Janus kinase-signal transducers and activators of transcription pathway were evaluated by reverse transcriptase polymerase chain reaction and western blot analysis. Interferon-γ increased angiotensinogen expression in mesangial cells with maximal augmentation observed following 5 ng/mL interferon-γ at 8 hours of treatment (1.87 ± 0.05, mRNA, relative ratio). Further increases were reduced or absent using higher concentrations of interferon-γ. Following treatments, monocyte chemoattractant protein 1 expression was induced in a linear dose-dependent manner (6.85 ± 0.62-fold by 20 ng/mL interferon-γ at 24 hours). In addition, interferon-γ induced STAT1 phosphorylation and suppressors of cytokine signaling 1 expression in a linear dose-dependent manner. The suppression of STAT1 and suppressors of cytokine signaling 1 expression by small interference RNAs facilitated an increase in interferon-γ-induced angiotensinogen expression, indicating that these two factors negatively regulate angiotensinogen expression. In contrast, the increase in interferon-γ-induced monocyte chemoattractant protein 1 expression was attenuated in STAT1-deficient mesangial cells, suggesting that STAT1 positively regulates monocyte chemoattractant protein 1 expression in mesangial cells. These results demonstrate that while interferon-γ increases both angiotensinogen and monocyte chemoattractant protein 1 expression, STAT1 plays an opposing role in the regulation of each factor in mesangial cells. |
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キーワード | 推奨 |
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発行所 | 推奨 | ||||||||||||||||||||||||||||||||||||||||||||||
誌名 | 必須 |
Journal of the Renin-Angiotensin-Aldosterone System : JRAAS(Sage Publication)
(pISSN: 1470-3203, eISSN: 1752-8976)
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巻 | 必須 | 21 | |||||||||||||||||||||||||||||||||||||||||||||
号 | 必須 | 3 | |||||||||||||||||||||||||||||||||||||||||||||
頁 | 必須 | 1470320320946527 1470320320946527 | |||||||||||||||||||||||||||||||||||||||||||||
都市 | 任意 | ||||||||||||||||||||||||||||||||||||||||||||||
年月日 | 必須 | 2020年 8月 3日 | |||||||||||||||||||||||||||||||||||||||||||||
URL | 任意 | ||||||||||||||||||||||||||||||||||||||||||||||
DOI | 任意 | 10.1177/1470320320946527 (→Scopusで検索) | |||||||||||||||||||||||||||||||||||||||||||||
PMID | 任意 | 32741247 (→Scopusで検索) | |||||||||||||||||||||||||||||||||||||||||||||
CRID | 任意 | ||||||||||||||||||||||||||||||||||||||||||||||
WOS | 任意 | ||||||||||||||||||||||||||||||||||||||||||||||
Scopus | 任意 | 2-s2.0-85088906311 | |||||||||||||||||||||||||||||||||||||||||||||
評価値 | 任意 | ||||||||||||||||||||||||||||||||||||||||||||||
被引用数 | 任意 | ||||||||||||||||||||||||||||||||||||||||||||||
指導教員 | 推奨 | ||||||||||||||||||||||||||||||||||||||||||||||
備考 | 任意 |
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