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著作: Komatsu Kei/Kumon Kento/Arita Mayuno/[鬼塚 正義]/[大政 健史]/Yohda Masafumi/Effect of the disulfide isomerase PDIa4 on the antibody production of Chinese hamster ovary cells./[Journal of Bioscience and Bioengineering]

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EID
373148
EOID
999038
Map
0
LastModified
2021年1月25日(月) 20:21:59
Operator
大家 隆弘
Avail
TRUE
Censor
0
Owner
鬼塚 正義
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨 研究
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Komatsu Kei
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Kumon Kento
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Arita Mayuno
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 鬼塚 正義([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 大政 健史
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Yohda Masafumi
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Effect of the disulfide isomerase PDIa4 on the antibody production of Chinese hamster ovary cells.

副題 任意
要約 任意

(英) Therapeutic monoclonal antibodies recognize and bind specific molecules on the surface of target cells, stimulating the immune system, which can attack these targeted cells. These antibodies are produced by mammalian cells, including Chinese hamster ovary (CHO) cells, because the formation of antibodies requires complicated posttranslational modifications, including peptidyl-prolyl cis/trans isomerization, disulfide bond formation, and glycosylation. Currently, it is thought that the efficient production of secretory proteins is limited by posttranslational processes. The ER is the biosynthesis site of all secreted and membrane proteins. The accumulation of unfolded proteins in the ER causes the ER stress response. During the ER stress state, various molecular chaperones are expressed to prevent proteins from the aggregate formation. The molecular chaperone involved in ER stress likely plays an essential role in the production of secretory proteins. The purpose of this study was to improve the production of monoclonal antibodies by cells. We elucidated the function of ER chaperones in the production of a monoclonal antibody. First, we quantitatively measured the mRNA expression levels of protein disulfide-isomerase family members. In CHO HcD6 cells treated with tunicamycin, the expression level of pdia4 was significantly increased. Second, we investigated the relationship between PDIa4 and antibody productivity in pdia4-knockdown cells. Both a decrease in the amount of secreted antibody and the accumulation of immature antibodies inside the cells were observed. Recombinant PDIa4 was able to refold the antibodies and Fabs. These results indicate that PDIa4 affects the production of monoclonal antibodies by catalyzing disulfide bond formation in these antibodies in CHO cells.

キーワード 推奨
  1. (英) Animals
  2. (英) Antibodies, Monoclonal
  3. (英) CHO Cells
  4. (英) Cricetinae
  5. (英) Cricetulus
  6. (英) Gene Expression Regulation, Enzymologic
  7. (英) Protein Disulfide-Isomerases
  8. (英) RNA, Messenger
発行所 推奨
誌名 必須 Journal of Bioscience and Bioengineering([日本生物工学会])
(pISSN: 1389-1723, eISSN: 1347-4421)
ISSN 任意 1347-4421
ISSN: 1389-1723 (pISSN: 1389-1723, eISSN: 1347-4421)
Title: Journal of bioscience and bioengineering
Title(ISO): J Biosci Bioeng
Supplier: 公益社団法人日本生物工学会
Publisher: Elsevier BV
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 130
必須 6
必須 637 643
都市 任意
年月日 必須 2020年 12月 初日
URL 任意
DOI 任意 10.1016/j.jbiosc.2020.08.001    (→Scopusで検索)
PMID 任意 32878739    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意 2-s2.0-85090018528
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: S1389-1723(20)30300-5

  2. (英) Article.ELocationID: 10.1016/j.jbiosc.2020.08.001

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article

  4. (英) KeywordList.Keyword: Antibody

  5. (英) KeywordList.Keyword: Chaperone

  6. (英) KeywordList.Keyword: Chinese hamster ovary

  7. (英) KeywordList.Keyword: Folding

  8. (英) KeywordList.Keyword: PDIa4

  9. (英) KeywordList.Keyword: Protein disulfide-isomerase