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著作: Kaneko Kei/Ohkawa Yuki/[橋本 登]/Ohmi Yuhsuke/Kotani Norihiro/Honke Koichi/Ogawa Mitsutaka/Okajima Tetsuya/Furukawa Keiko/Furukawa Koichi/Neogenin, Defined as a GD3-associated Molecule by Enzyme-mediated Activation of Radical Sources, Confers Malignant Properties via Intracytoplasmic Domain in Melanoma Cells./[The Journal of Biological Chemistry]

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EID
370986
EOID
990932
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LastModified
2020年9月2日(水) 18:43:12
Operator
橋本 登
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橋本 登
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Kaneko Kei
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Ohkawa Yuki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 橋本 登([徳島大学.大学院医歯薬学研究部.歯学域.先端歯学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.組織再生制御学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Ohmi Yuhsuke
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Kotani Norihiro
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Honke Koichi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Ogawa Mitsutaka
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. (英) Okajima Tetsuya
    役割 任意
    貢献度 任意
    学籍番号 推奨
  9. (英) Furukawa Keiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  10. (英) Furukawa Koichi
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Neogenin, Defined as a GD3-associated Molecule by Enzyme-mediated Activation of Radical Sources, Confers Malignant Properties via Intracytoplasmic Domain in Melanoma Cells.

副題 任意
要約 任意

(英) To investigate mechanisms for increased malignant properties in malignant melanomas by ganglioside GD3, enzyme-mediated activation of radical sources and subsequent mass spectrometry were performed using an anti-GD3 antibody and GD3-positive (GD3+) and GD3-negative (GD3-) melanoma cell lines. Neogenin, defined as a GD3-neighbored molecule, was largely localized in lipid/rafts in GD3+ cells. Silencing of neogenin resulted in the reduction of cell growth and invasion activity. Physical association between GD3 and neogenin was demonstrated by immunoblotting of the immunoprecipitates with anti-neogenin antibody from GD3+ cell lysates. The intracytoplasmic domain of neogenin (Ne-ICD) was detected in GD3+ cells at higher levels than in GD3- cells when cells were treated by a proteasome inhibitor but not when simultaneously treated with a γ-secretase inhibitor. Exogenous GD3 also induced increased Ne-ICD in GD3- cells. Overexpression of Ne-ICD in GD3- cells resulted in the increased cell growth and invasion activity, suggesting that Ne-ICD plays a role as a transcriptional factor to drive malignant properties of melanomas after cleavage with γ-secretase. γ-Secretase was found in lipid/rafts in GD3+ cells. Accordingly, immunocyto-staining revealed that GD3, neogenin, and γ-secretase were co-localized at the leading edge of GD3+ cells. All these results suggested that GD3 recruits γ-secretase to lipid/rafts, allowing efficient cleavage of neogenin. ChIP-sequencing was performed to identify candidates of target genes of Ne-ICD. Some of them actually showed increased expression after expression of Ne-ICD, probably exerting malignant phenotypes of melanomas under GD3 expression.

キーワード 推奨
  1. (英) Amyloid Precursor Protein Secretases
  2. (英) Cell Line, Tumor
  3. (英) Gangliosides
  4. (英) Gene Expression Regulation, Neoplastic
  5. (英) Humans
  6. (英) Melanoma
  7. (英) Membrane Microdomains
  8. (英) Neoplasm Proteins
  9. (英) Nerve Tissue Proteins
  10. (英) Receptors, Cell Surface
発行所 推奨
誌名 必須 The Journal of Biological Chemistry([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)
ISSN 任意 1083-351X
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 291
必須 32
必須 16630 16643
都市 任意
年月日 必須 2016年 6月 10日
URL 任意
DOI 任意 10.1074/jbc.M115.708834    (→Scopusで検索)
PMID 任意 27288875    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) PublicationType: Journal Article

  2. (英) PublicationType: Research Support, Non-U.S. Gov't