著作: [福田 達也]/中谷 奈津/[米田 晋太朗]/[小暮 健太朗]/Weak electric current treatment to artificially enhance vascular permeability in embryonated chicken eggs/[Biological & Pharmaceutical Bulletin]
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種別 | 必須 | 学術論文(審査論文) | |||
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言語 | 必須 | 英語 | |||
招待 | 推奨 | ||||
審査 | 推奨 | Peer Review | |||
カテゴリ | 推奨 | 研究 | |||
共著種別 | 推奨 | 学内共著(徳島大学内研究者との共同研究 (学外研究者を含まない)) | |||
学究種別 | 推奨 | 学士課程学生による研究報告 | |||
組織 | 推奨 | ||||
著者 | 必須 | ||||
題名 | 必須 |
(英) Weak electric current treatment to artificially enhance vascular permeability in embryonated chicken eggs |
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副題 | 任意 | ||||
要約 | 任意 |
(英) Technologies that overcome the barrier presented by vascular endothelial cells are needed to facilitate targeted delivery of drugs into tissue parenchyma by intravenous administration. We previously reported that weak electric current treatment (ET: 0.3-0.5 mA/cm) applied onto skin tissue in a transdermal drug delivery technique termed iontophoresis induces cleavage of intercellular junctions that results in permeation of macromolecules such as small interfering RNA and cytosine-phosphate-guanine (CpG) oligonucleotide through the intercellular space. Based on these findings, we hypothesized that application of ET to blood vessels could promote cleavage of intercellular junctions that artificially induces increase in vascular permeability to enhance extravasation of drugs from the vessels into target tissue parenchyma. Here we investigated the effect of ET (0.34 mA/cm) on vascular permeability using embryonated chicken eggs, which have blood vessels in the chorioallantoic membrane (CAM), as an animal model. ET onto the CAM of the eggs significantly increased extravasation of intravenously injected calcein (M.W. 622.6), a low molecular weight compound model, and the macromolecule fluorescein isothiocyanate (FITC)-dextran (M.W. 10000). ET-mediated promotion of penetration of FITC-dextran through vascular endothelial cells was also observed in transwell permeability assay using monolayer of human umbilical vein endothelial cells without induction of obvious cellular damage. Confocal microscopy detected remarkable fluorescence derived from injected FITC-dextran in blood vessel walls. These results in embryonated chicken eggs suggest that ET onto blood vessels could artificially enhance vascular permeability to facilitate extravasation of macromolecules from blood vessels. |
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キーワード | 推奨 |
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発行所 | 推奨 | ||||
誌名 | 必須 |
Biological & Pharmaceutical Bulletin([日本薬学会])
(pISSN: 0918-6158, eISSN: 1347-5215)
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巻 | 必須 | 43 | |||
号 | 必須 | 11 | |||
頁 | 必須 | 1729 1734 | |||
都市 | 任意 | ||||
年月日 | 必須 | 2020年 11月 初日 | |||
URL | 任意 | ||||
DOI | 任意 | 10.1248/bpb.b20-00423 (→Scopusで検索) | |||
PMID | 任意 | 33132318 (→Scopusで検索) | |||
CRID | 任意 | ||||
WOS | 任意 | ||||
Scopus | 任意 | 2-s2.0-85095385288 | |||
評価値 | 任意 | ||||
被引用数 | 任意 | ||||
指導教員 | 推奨 | ||||
備考 | 任意 |
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