徳島大学 教育・研究者情報データベース(EDB)

Education and Research Database (EDB), Tokushima University

徳島大学ウェブサイトへのリンク

(EDB発行のIDとパスフレーズ,又は情報センター発行の個人cアカウントとパスワードでログインしてください.)

著作: [福田 達也]/西川 明菜/[小暮 健太朗]/Low level electricity increases the secretion of extracellular vesicles from cultured cells/[Biochemistry and Biophysics Reports]

ヘルプを読む

「著作」(著作(著書,論文,レター,国際会議など))は,研究業績にかかる著作(著書,論文,レター,国際会議など)を登録するテーブルです. (この情報が属するテーブルの詳細な定義を見る)

  • 項目名の部分にマウスカーソルを置いて少し待つと,項目の簡単な説明がツールチップ表示されます.

この情報をEDB閲覧画面で開く

EID
361357
EOID
978590
Map
0
LastModified
2020年4月21日(火) 18:33:38
Operator
大家 隆弘
Avail
TRUE
Censor
0
Owner
小暮 健太朗
Read
継承
Write
継承
Delete
継承
種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 福田 達也
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Nishikawa Akina / (日) 西川 明菜 / (読) にしかわ あきな
    役割 任意
    貢献度 任意
    学籍番号 推奨 ****
  3. 小暮 健太朗([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.衛生薬学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Low level electricity increases the secretion of extracellular vesicles from cultured cells

副題 任意
要約 任意

(英) Exosomes, a type of extracellular vesicles, can be collected from the conditioned medium of cultured cells, and are expected to be used in disease therapy and drug delivery systems. However, since the yield of exosomes from conditioned medium is generally low, investigations to develop new methods to increase exosome secretion and to elucidate the secretion mechanism have been performed. Our previous studies demonstrated that activation of intracellular signaling including Rho GTPase and subsequent endocytosis of extraneous molecules in cells could be induced by low level electricity (0.3-0.5 mA/cm). Since exosomes are produced in the process of endocytosis and secreted by exocytosis via certain signaling pathways, we hypothesized that low level electric treatment (ET) would increase exosome secretion from cultured cells via intracellular signaling activation. In the present study, the influence of ET (0.34 mA/cm) on extracellular vesicle (EV) secretion from cultured cells was examined by using murine melanoma and murine fibroblast cells. The results showed that the number of EV particles collected by ultracentrifugation was remarkably increased by ET in both cell lines without cellular toxicity or changes in the particle distribution. Also, protein amounts of the collected EVs were significantly increased in both cells by ET without alteration of expression of representative exosome marker proteins. Moreover, in both cells, the ratio of particle numbers to protein amount was not significantly changed by ET. Rho GTPase inhibition significantly suppressed ET-mediated increase of EV secretion in murine melanoma, indicating that Rho GTPase activation could be involved in ET-mediated EV secretion in the cell. Additionally, there were almost no differences in uptake of each EV into each donor cell regardless of whether the cells had been exposed to ET for EV collection. Taken together, these results suggest that ET could increase EV secretion from both cancer and normal cells without apparent changes in EV quality.

キーワード 推奨
発行所 推奨
誌名 必須 Biochemistry and Biophysics Reports(Elsevier B.V.)
(eISSN: 2405-5808)
ISSN 任意 2405-5808
ISSN: 2405-5808 (eISSN: 2405-5808)
Title: Biochemistry and biophysics reports
Title(ISO): Biochem Biophys Rep
Publisher: Elsevier B.V.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 21
必須 ---
必須 100713 100713
都市 任意
年月日 必須 2020年 3月 初日
URL 任意
DOI 任意 10.1016/j.bbrep.2019.100713    (→Scopusで検索)
PMID 任意 31828227    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1016/j.bbrep.2019.100713

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) KeywordList.Keyword: Exosome secretion

  4. (英) KeywordList.Keyword: Exosomes

  5. (英) KeywordList.Keyword: Extracellular vesicles

  6. (英) KeywordList.Keyword: Intracellular signaling

  7. (英) KeywordList.Keyword: Low level electric treatment