徳島大学 教育・研究者情報データベース(EDB)

1. 安藝 健作([徳島大学.大学院医歯薬学研究部.保健学域.保健科学部門.医用検査学系.細胞・免疫解析学])

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2. (日) 佐藤 瑞樹

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3. (英) Sone Atsumi / (日) 曽根 淳美

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4. 川添 和義

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5. 細井 英司

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(英) Evaluation of NK cell function using human NK-like cultured cell, KHYG-1

(日) ヒトNK様培養細胞KHYG-1を用いたNK細胞機能の評価

(日) NK cells play an important role in the elimination of viral infection and tumors. In recent years, immune cell therapy using activated NK cells has attracted attention, and the search for activators and evaluation of NK cell activity have become important. We previously reported a positive correlation between CD56antigen expression, which is the major cell membrane antigen of human NK cells, and NK cell activity or cytotoxicity, and demonstrated that it is possible to evaluate NK cell function using the CD56 antigen as an index. This simple evaluation method is useful for functional evaluation of NK cells and the search for activators. However, it requires blood sampling and preparation of NK cells because it uses human NK cells. Therefore, in this study, we examined whether the CD56 antigen functions as an activation index using KHYG‐1 human NK-like cultured cells as a substitute for NK cells. As a result, the CD56antigen on the KHYG‐1cell membrane was increased in a concentration-dependent manner by IL‐2 stimulation, as was the cytotoxicity. This suggests that the CD56 antigen on the KHYG‐1 cell membrane can be used as an evaluation index of NK activity as in human NK cells.

1. (英) NK cell
2. (英) KHYG-1
3. (英) CD56 antigen
4. (英) IL-2

1. 細井 英司