徳島大学 教育・研究者情報データベース(EDB)

1. (英) Nonaka Kohei / (日) 野中 康平 / (読) のなか こうへい

   役割	任意
   貢献度	任意
   学籍番号	推奨	****

2. 梶浦 由加里

   役割	任意
   貢献度	任意
   学籍番号	推奨

3. 板東 美香([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学]/[徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])

   役割	任意
   貢献度	任意
   学籍番号	推奨

4. 坂本 英次郎

   役割	任意
   貢献度	任意
   学籍番号	推奨

5. 稲垣 裕司([徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])

   役割	任意
   貢献度	任意
   学籍番号	推奨

6. (英) Lew JH

   役割	任意
   貢献度	任意
   学籍番号	推奨

7. 成石 浩司

   役割	任意
   貢献度	任意
   学籍番号	推奨

8. 生田 貴久

   役割	任意
   貢献度	任意
   学籍番号	推奨

9. 吉田 賀弥([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健支援学]/[徳島大学.歯学部.口腔保健学科.口腔保健支援学講座])

   役割	任意
   貢献度	任意
   学籍番号	推奨

10. (英) Kobayashi Tesuo

    役割	任意
    貢献度	任意
    学籍番号	推奨

11. (英) Yoshie Hiromasa

    役割	任意
    貢献度	任意
    学籍番号	推奨

12. 永田 俊彦

    役割	任意
    貢献度	任意
    学籍番号	推奨

13. 木戸 淳一

    役割	任意
    貢献度	任意
    学籍番号	推奨

(英) Advanced glycation end-products increase IL-6 and ICAM-1 expression via RAGE, MAPK and NF-kB pathways in human gingival fibroblasts

(英) Diabetes mellitus (DM) is a risk factor for periodontal diseases and may exacerbate the progression of the pathogenesis of periodontitis. Advanced glycation end-products (AGEs) cause DM complications relative to levels of glycemic control and larger amounts accumulate in the periodontal tissues of patients with periodontitis and DM. In the present study, we investigated the effects of AGEs on the expression of inflammation-related factors in human gingival fibroblasts (HGFs) to elucidate the impact of AGEs on DM-associated periodontitis. HGFs were cultured with or without AGEs. Cell viability was examined, and RNA and protein fractions were isolated from AGE-treated cells. The expression of interleukin (IL)-6, intercellular adhesion molecule-1 (ICAM-1), and the receptor for AGE (RAGE) was investigated using reverse transcription-polymerase chain reaction, quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, and reactive oxygen species activity was measured using a kit with 2',7'-dichlorofluorescin diacetate. Human monocytic cells (THP-1) labeled with a fluorescent reagent were co-cultured with HGFs treated with AGEs and IL-6 siRNA, and the adhesive activity of THP-1 cells to HGFs was assessed. The expression of IL-6 and ICAM-1 was examined when HGFs were pretreated with recombinant human IL-6, the siRNAs of RAGE and IL-6, and inhibitors of MAPK and NF-κB, and then cultured with and without AGEs. The phosphorylation of MAPK and NF-κB was assessed using western blotting. AGEs increased the mRNA and protein expressions of RAGE, IL-6, ICAM-1 and reactive oxygen species activity in HGFs, and promoted the adhesion of THP-1 cells to HGFs, but had no effect on cell viability until 72 hours. Recombinant human IL-6 increased ICAM-1 expression in HGFs, while the siRNAs of RAGE and IL-6 inhibited AGE-induced IL6 and ICAM1 mRNA expression, and IL-6 siRNA depressed AGE-induced THP-1 cell adhesion. AGEs increased the phosphorylation of p38 and ERK MAPKs, p65 NF-κB and IκBα, while inhibitors of p38, ERK MAPKs and NF-κB significantly decreased AGE-induced IL-6 and ICAM-1 expression. AGEs increase IL-6 and ICAM-1 expression via the RAGE, MAPK and NF-κB pathways in HGFs and may exacerbate the progression of the pathogenesis of periodontal diseases.

1. (英) Antigens, Neoplasm
2. (英) Diabetes Complications
3. (英) Fibroblasts
4. (英) Gingiva
5. (英) Glycation End Products, Advanced
6. (英) Humans
7. (英) Intercellular Adhesion Molecule-1
8. (英) Interleukin-6
9. (英) MAP Kinase Signaling System
10. (英) Mitogen-Activated Protein Kinase Kinases
11. (英) Mitogen-Activated Protein Kinases
12. (英) NF-kappa B
13. (英) Periodontitis
14. (英) Phosphorylation
15. (英) Reactive Oxygen Species
16. (英) THP-1 Cells

1. (英) Article.ELocationID: 10.1111/jre.12518

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) KeywordList.Keyword: ICAM-1

4. (英) KeywordList.Keyword: Interleukin-6

5. (英) KeywordList.Keyword: advanced glycation end-product

6. (英) KeywordList.Keyword: human gingival fibroblasts