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著作: Tanimoto Keiji/Matsuzaki Hitomi/[岡村 永一]/Ushiki Aki/Fukamizu Akiyoshi/Engel James Douglas/Transvection-like interchromosomal interaction is not observed at the transcriptional level when tested in the Rosa26 locus in mouse./[PLoS ONE]

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EID
349526
EOID
939186
Map
0
LastModified
2019年2月21日(木) 16:13:16
Operator
岡村 永一
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TRUE
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岡村 永一
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種別 必須
言語 必須 英語
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著者 必須
  1. (英) Tanimoto Keiji
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    学籍番号 推奨
  2. (英) Matsuzaki Hitomi
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  3. 岡村 永一
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  4. (英) Ushiki Aki
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    学籍番号 推奨
  5. (英) Fukamizu Akiyoshi
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    学籍番号 推奨
  6. (英) Engel James Douglas
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題名 必須

(英) Transvection-like interchromosomal interaction is not observed at the transcriptional level when tested in the Rosa26 locus in mouse.

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(英) Long-range associations between enhancers and their target gene promoters have been shown to play critical roles in executing genome function. Recent variations of chromosome capture technology have revealed a comprehensive view of intra- and interchromosomal contacts between specific genomic sites. The locus control region of the β-globin genes (β-LCR) is a super-enhancer that is capable of activating all of the β-like globin genes within the locus in cis through physical interaction by forming DNA loops. CTCF helps to mediate loop formation between LCR-HS5 and 3'HS1 in the human β-globin locus, in this way thought to contribute to the formation of a "chromatin hub". The β-globin locus is also in close physical proximity to other erythrocyte-specific genes located long distances away on the same chromosome. In this case, erythrocyte-specific genes gather together at a shared "transcription factory" for co-transcription. Theoretically, enhancers could also activate target gene promoters at the identical loci, yet on different chromosomes in trans, a phenomenon originally described as transvection in Drosophilla. Although close physical proximity has been reported for the β-LCR and the β-like globin genes when integrated at the mouse homologous loci in trans, their structural and functional interactions were found to be rare, possibly because of a lack of suitable regulatory elements that might facilitate such trans interactions. Therefore, we re-evaluated presumptive transvection-like enhancer-promoter communication by introducing CTCF binding sites and erythrocyte-specific transcription units into both LCR-enhancer and β-promoter alleles, each inserted into the mouse ROSA26 locus on separate chromosomes. Following cross-mating of mice to place the two mutant loci at the identical chromosomal position and into active chromation in trans, their transcriptional output was evaluated. The results demonstrate that there was no significant functional association between the LCR and the β-globin gene in trans even in this idealized experimental context.

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誌名 必須 PLoS ONE(Public Library of Science)
(eISSN: 1932-6203)
ISSN 任意 1932-6203
ISSN: 1932-6203 (eISSN: 1932-6203)
Title: PloS one
Title(ISO): PLoS One
Publisher: PLOS
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 14
必須 2
必須
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年月日 必須 2019年 2月 14日
URL 任意
DOI 任意 10.1371/journal.pone.0203099    (→Scopusで検索)
PMID 任意 30763343    (→Scopusで検索)
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  1. (英) PublicationType: Journal Article