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著作: [辻 明彦]/[湯浅 恵造]/[浅田 元子]/Cellulose-binding activity of a 21-kDa endo-ß-1,4-glucanase lacking cellulose-binding domain and its synergy with other cellulases in the digestive fluid of Aplysia kurodai/[PLoS ONE]

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EID
347340
EOID
957007
Map
0
LastModified
2019年8月19日(月) 18:29:08
Operator
大家 隆弘
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TRUE
Censor
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Owner
辻 明彦
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
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学究種別 推奨
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著者 必須
  1. 辻 明彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 湯浅 恵造
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 浅田 元子([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
    役割 任意
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    学籍番号 推奨
題名 必須

(英) Cellulose-binding activity of a 21-kDa endo-ß-1,4-glucanase lacking cellulose-binding domain and its synergy with other cellulases in the digestive fluid of Aplysia kurodai
副題 任意
要約 任意

(英) Endo-ß-1,4-glucanase AkEG21 belonging to glycosyl hydrolase family 45 (GHF45) is the most abundant cellulase in the digestive fluid of sea hare (Aplysia kurodai). The specific activity of this 21-kDa enzyme is considerably lower than those of other endo ß-1,4-glucanases in the digestive fluid of A. kurodai, therefore its role in whole cellulose hydrolysis by sea hare is still uncertain. Although AkEG21 has a catalytic domain without a cellulose binding domain, it demonstrated stable binding to cellulose fibers, similar to that of fungal cellobiohydrolase (CBH) 1 and CBH 2, which is strongly inhibited by cellohexaose, suggesting the involvement of the catalytic site in cellulose binding. Cellulose-bound AkEG21 hydrolyzed cellulose to cellobiose, cellotriose and cellotetraose, but could not digest an external substrate, azo-carboxymethyl cellulose. Cellulose hydrolysis was considerably stimulated by the synergistic action of cellulose-bound AkEG21 and AkEG45, another ß-1,4-endoglucanase present in the digestive fluid of sea hare; however no synergy in carboxymethylcellulose hydrolysis was observed. When AkEG21 was removed from the digestive fluid by immunoprecipitation, the cellulose hydrolyzing activity of the fluid was significantly reduced, indicating a critical role of AkEG21 in cellulose hydrolysis by A. kurodai. These findings suggest that AkEG21 is a processive endoglucanase functionally equivalent to the CBH, which provides a CBH-independent mechanism for the mollusk to digest seaweed cellulose to glucose.
キーワード 推奨
  1. (英) Animals
  2. (英) Aplysia
  3. (英) Catalytic Domain
  4. (英) Cellobiose
  5. (英) Cellulase
  6. (英) Cellulose
  7. (英) Digestion
  8. (英) Glucose
  9. (英) Hydrolysis
  10. (英) Kinetics
  11. (英) Oligosaccharides
  12. (英) Protein Binding
  13. (英) Protein Domains
  14. (英) Tetroses
発行所 推奨
誌名 必須 PLoS ONE(Public Library of Science)
(eISSN: 1932-6203)
ISSN 任意 1932-6203
ISSN: 1932-6203 (eISSN: 1932-6203)
Title: PloS one
Title(ISO): PLoS One
Publisher: PLOS
 (NLM Catalog)   (Scopus)   (CrossRef)  (Scopus information is found. [need login])
巻 必須 13
号 必須 11
頁 必須 e0205915 e0205915
都市 任意
年月日 必須 2018年 11月 9日
URL 任意
DOI 任意 10.1371/journal.pone.0205915    (→Scopusで検索)
PMID 任意 30412581    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意 2-s2.0-85056302997
機関リポジトリ 112918
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意

  1. (英) Article.ELocationID: 10.1371/journal.pone.0205915

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) CoiStatement: The authors have declared that no competing interests exist.