著作: [真板 宣夫]/Crystal Structure Determination of Ubiquitin by Fusion to a Protein That Forms a Highly Porous Crystal Lattice./[Journal of the American Chemical Society]
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削除する| 種別 | 必須 | 学術レター(ショートペーパー) | |||||||||
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| 言語 | 必須 | 英語 | |||||||||
| 招待 | 推奨 | ||||||||||
| 審査 | 推奨 | Peer Review | |||||||||
| カテゴリ | 推奨 | 研究 | |||||||||
| 共著種別 | 推奨 | 単独著作(徳島大学内の単一の研究グループ(研究室等)内の研究 (単著も含む)) | |||||||||
| 学究種別 | 推奨 | ||||||||||
| 組織 | 推奨 | ||||||||||
| 著者 | 必須 |
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| 題名 | 必須 |
(英) Crystal Structure Determination of Ubiquitin by Fusion to a Protein That Forms a Highly Porous Crystal Lattice. |
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| 副題 | 任意 | ||||||||||
| 要約 | 任意 |
(英) The protein crystallization process requires screening of a large number of conditions using a large quantity of high-purity protein, which makes crystal structure analysis difficult. Thus, the development of easy and versatile protein crystallization techniques is both extremely desirable and highly challenging. Here I demonstrate the crystallization and structure determination of ubiquitin by genetic fusion to the highly porous honeycomb lattice of R1EN. I successfully crystallized and collected X-ray data from three R1EN-ubiquitin constructs with various linker lengths under the same conditions as the original R1EN. The crystals diffracted to 1.7-2.4 Å resolution, and the ubiquitin structures were determined with results almost identical to the previously published structure. Moreover, the ubiquitin structure could be solved by molecular replacement using R1EN alone. This method may reduce the effort required for crystallization screening and is applicable to de novo protein structure determination. |
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| キーワード | 推奨 | ||||||||||
| 発行所 | 推奨 | ||||||||||
| 誌名 | 必須 |
Journal of the American Chemical Society([アメリカ化学会])
(pISSN: 0002-7863, eISSN: 1520-5126)
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| 巻 | 必須 | 140 | |||||||||
| 号 | 必須 | 42 | |||||||||
| 頁 | 必須 | 13546 13549 | |||||||||
| 都市 | 任意 | ||||||||||
| 年月日 | 必須 | 2018年 10月 24日 | |||||||||
| URL | 任意 | ||||||||||
| DOI | 任意 | 10.1021/jacs.8b07512 (→Scopusで検索) | |||||||||
| PMID | 任意 | 30299944 (→Scopusで検索) | |||||||||
| CRID | 任意 | ||||||||||
| WOS | 任意 | ||||||||||
| Scopus | 任意 | ||||||||||
| 機関リポジトリ | 112910 | ||||||||||
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| 指導教員 | 推奨 | ||||||||||
| 備考 | 任意 |
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