徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.総合科学教育部.地域科学専攻
2. 徳島大学.総合科学部.社会創生学科.環境共生コース(2009年4月1日〜2024年3月31日)
3. 徳島大学.生物資源産業学部.生物資源産業学科.食料科学コース(2016年4月1日〜)

1. (英) Funakoshi Yurie / (日) 舟越 裕里江 / (読) ふなこし ゆりえ

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2. (英) Azuma Ayako / (日) 東 亜弥子 / (読) あずま あやこ

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3. (英) Ishikawa Mizuki / (日) 石川 みづき / (読) いしかわ みづき

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4. (英) Itsuki Satoru / (日) 伊槻 悟 / (読) いつき さとる

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5. (英) Tamura Yasuaki / (日) 田村 靖明 / (読) たむら やすあき

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6. 金丸 芳

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7. 平井 松午

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8. 小山 保夫

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(英) Cytometrical analysis of the adverse effects of indican, indoxyl, indigo, and indirubin on rat thymic lymphocytes

(英) Many businesses thrive by producing health supplements from agricultural products, as exemplified by the production of functional (or health) food using plants traditionally cultivated in the rural areas. Dyes, such as indican, indigo, indoxyl, and indirubin, present in dye plants, possess antibacterial, antifungal, and antiproliferative activities. However, these effects may also lead to cytotoxicity. Thus, studies in normal mammalian cells are necessary to identify cytotoxicity and prevent adverse effects of functional foods that contain these dyes. In this study, the effects of indican, indigo, indoxyl, and indirubin were evaluated by flow cytometry using appropriate fluorescent probes in rat thymic lymphocytes. Among the dyes analyzed, indirubin exerted distinct cellular activities. Treatment with indirubin (10 30 µM) increased the population of shrunken dead cells. The side scatter, but not forward scatter, increased in indirubin-treated living cells. It increased the population of annexin V-bound living and dead cells and that of dead cells without annexin V. Indirubin elevated intracellular Ca2+, but not Zn2+ levels. The cellular content of superoxide anions and increased glutathione decreased. Indirubin depolarized the cellular plasma and mitochondrial membranes. It did not potentiate or attenuate the cytotoxicity of A23187 (Ca2+ overload) and H2O2 (oxidative stress). The results suggested that indirubin induces both apoptotic and non-apoptotic cell death. It may be difficult to predict and prevent adverse effects of indirubin due to its diverse activities on normal mammalian cells. Therefore, indirubin should be removed from products that contain dye plant extracts.

1. (英) Indigo
2. (英) Indirubin
3. (英) Indoxyl
4. (英) Indican