徳島大学 教育・研究者情報データベース(EDB)

1. (英) Hatakeyama Tomomitsu

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2. (英) Ichise Ayaka

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3. (英) Unno Hideaki

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4. 郷田 秀一郎([長崎大学])

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5. (英) Oda Tatsuya

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6. (英) Tateno Hiroaki

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7. (英) Hirabayashi Jun

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8. 酒井 仁美([徳島大学.技術支援部.常三島技術部門.地域協働グループ])

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9. 中川 秀幸

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(英) Carbohydrate recognition by the rhamnose-binding lectin SUL-I with a novel three-domain structure isolated from the venom of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus

(英) The globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus contains several biologically active proteins. We have cloned the cDNA of one of the toxin components, SUL-I, which is a rhamnose-binding lectin (RBL) that acts as a mitogen through binding to carbohydrate chains on target cells. Recombinant SUL-I (rSUL-I) was produced in Escherichia coli cells, and its carbohydrate-binding specificity was examined with the glycoconjugate microarray analysis, which suggested that potential target carbohydrate structures are galactose-terminated N-glycans. rSUL-I exhibited mitogenic activity for murine splenocyte cells and toxicity against Vero cells. The three-dimensional structure of the rSUL-I/l-rhamnose complex was determined by X-ray crystallographic analysis at a 1.8 Å resolution. The overall structure of rSUL-I is composed of three distinctive domains with a folding structure similar to those of CSL3, a RBL from chum salmon (Oncorhynchus keta) eggs. The bound l-rhamnose molecules are mainly recognized by rSUL-I through hydrogen bonds between its 2-, 3-, and 4-hydroxy groups and Asp, Asn, and Glu residues in the binding sites, while Tyr and Ser residues participate in the recognition mechanism. It was also inferred that SUL-I may form a dimer in solution based on the molecular size estimated via dynamic light scattering as well as possible contact regions in its crystal structure.

1. (英) Amino Acid Sequence
2. (英) Animal Structures
3. (英) Animals
4. (英) Binding Sites
5. (英) Carbohydrate Sequence
6. (英) Cercopithecus aethiops
7. (英) Cloning, Molecular
8. X線結晶学(X-ray crystallography)
9. 大腸菌(Escherichia coli)
10. 遺伝子発現(gene expression)
11. (英) Hydrogen Bonding
12. (英) Lectins
13. (英) Lymphocytes
14. (英) Marine Toxins
15. (英) Mice
16. (英) Microarray Analysis
17. (英) Mitogens
18. (英) Models, Molecular
19. (英) Polysaccharides
20. (英) Protein Binding
21. (英) Protein Interaction Domains and Motifs
22. (英) Protein Multimerization
23. (英) Protein Structure, Secondary
24. (英) Recombinant Proteins
25. (英) Rhamnose
26. (英) Sea Urchins
27. (英) Vero Cells

1. (英) Article.ELocationID: 10.1002/pro.3185

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) KeywordList.Keyword: X-ray crystallographic analysis

4. (英) KeywordList.Keyword: lectin

5. (英) KeywordList.Keyword: rhamnose

6. (英) KeywordList.Keyword: sea urchin

7. (英) KeywordList.Keyword: toxin